Meeting the orphan: early relational trauma, synchronicity and the psychoid.

In this paper I attempt to show how deep engagement, which is gradually developed within the relational container, can facilitate access to the psychoid dimension of the unconscious characterized by a quality of interconnectedness. The resulting synchronistic experiences can support and strengthen this container as well as having profound re-connecting impact on the people involved. With this in mind, I suggest that openness to this dimension can be very important in working with the issues of loss, abandonment, aloneness and mistrust in human connections - the symbolic domain of the orphan.

[Occupational therapy: achievements and problems].

Occupational therapy is topical and attracts attention of various medical specialists, for being on junction between clinical, hygienic, social and economical sciences. The authors present major directions of research and practical work of the Institute from its foundation till nowadays.

[Features of anesthesia in rats at abdominal operations].

Nowadays, in Russia, diethyl ether is the most popular narcosis for rodent and particular rats. We had tested the new methods based on Zoletil 100 + XylaVet (15 mg/kg and 15-10-5 mg/kg) respectively. 6 conventional female rats were treated with this narcosis.

Addressing psychological aspects of physical problems through sandplay: a grounded theory study of therapists' views.

Objectives: This study aimed to explore therapists' understanding of how people with a wide range of physical problems address the psychological aspects of these problems through sandplay, what happens for them in the process, what changes they experience and what sandplay can contribute to working with such people.

Design: This exploratory qualitative study used grounded theory to systematically analyse the data and construct a substantive theory of therapists' understanding of the processes and themes involved in sandplay therapy with people with physical problems.

Methods: Semi-structured interviews were carried out with nine sandplay therapists with the participants asked about their experiences of using sandplay to address physical problems.

[Liver defect and change of tolerance to low alcohol drinks at young rats].

This study was performed to understand behavior and toxic liver defect in rats in the use of low-alcohol drinks (LAD) and energy drinks (ED). Young rats quickly become accustomed to the LAD, increased tolerance to alcohol. Rats were getting a habit, an intoxicating effect was faster than at the beginning of the LAD using.

[Parameters of the succinate transport into Saccharomices cerevisiae cells after prolonged cold preincubation].

A nonconventional approach to the measurement of succinate transport through plasmalemma is proposed. It is based on the conditions in which the succinate oxidation rate is limited by transport through plasmalemma. Impermeable specific inhibitor of plasmalemma dicarboxylate transporter was employed as a tool to optimize conditions for the transport activity assay.

Regulation of c-Fos gene expression in the rat olfactory bulb during olfactory learning.

An immunohistochemical method was used to study the expression of transcription factor c-Fos in the mid part of the main olfactory bulbs in 18-day-old rats after training to seek their mothers using an olfactory orientation cue (propionic acid) in a Y-maze. On the next day, rat pups demonstrated a significant preference for the propionic acid odor in behavioral tests, as compared with control animals trained to the skill without the olfactory orientation cue and pups familiarized with the odor without forming any association between it and any kind of reinforcement. The propionic acid odor evoked an insignificant increase in c-Fos expression, predominantly in the granular layer of the dorsomedial area of the olfactory bulb.

Comparative analysis of dipsogenic effects of systemic and intracerebral injection of angiotensin II to rats after carotid glomectomy.

Systemic administration of angiotensin II after carotid glomectomy produced a less pronounced dipsogenic effects (consumption of water and NaCl solution) compared to sham-operated control animals. Injection of angiotensin II into the lateral cerebral ventricles of the same glomectomized rats increased water and NaCl consumption to a level surpassing that of sham-operated animals. The number of drinking acts and comfortable grooming acts decreased in glomectomized animals after systemic administration of angiotensin II, but increased after its intracerebral injection compared to the control.

[Regulation of c-Fos expression in the rat olfactory bulbs during olfactory learning].

The distribution of c-Fos-immunopositive neurons was examined in the mitral/tufted and granular cell layers in the medium part of the main olfactory bulbs of 18-day-old rats after they had been trained for propionic acid vapour-guided search for dam in the Y-maze. On the next day these pups exhibited a strong preference for the propionic acid odor as compared to the control pups trained for this task without the odor cue and odor-familiarized pups exposed to propionic acid as a novel neutral stimulus. Exposure to propionic acid produced a moderate activation of c-Fos expression, mainly in the granular layer of the dorsomedial part of the bulb.

Specific features of changes in levels of endogenous respiration substrates in Saccharomyces cerevisiae cells at low temperature.

The rate of endogenous respiration of Saccharomyces cerevisiae cells incubated at 0 degrees C under aerobic conditions in the absence of exogenous substrates decreased exponentially with a half-period of about 5 h when measured at 30 degrees C. This was associated with an indirectly shown decrease in the level of oxaloacetate in the mitochondria in situ. The initial concentration of oxaloacetate significantly decreased the activity of succinate dehydrogenase.

[Isolation of cell membranes from Saccharomyces cerevisiae for evaluation of the protein composition].

We describe a simple method for the isolation of membrane fractions from Saccharomyces cerevisiae yeasts, containing the complex of plasma membranes and cell walls. The method is based on cell disruption on an INBI flow disintegrator. This device spares subcellular structures, which simplifies the isolation of cell membranes.

[Turbidimetric method for quantitative determination of triton X-100 with silicotungstic acid].

The Formation of Triton X-100-silicotungstic acid complex was studied. Quantitative turbidimetric determination of the detergent based on this process was suggested. This method allows to determining the complex formation at any wavelength in the range from 350 (epsilon 350 = 15,600 cm-1 M-1) to 600 nm (epsilon 600 = = 9090 cm-1 M-1).

An equation for the reconstitution of membrane proteins and a method for determination of pore content in biological membranes.

The dependence between specific trapped volume of liposomes (W) and protein concentration (p) is proposed to be used for quantitative pore determination in biological membranes via pore reconstitution into liposomes. This dependence is described by the following equation: p = -p(e) x ln(W/W0), where W0 is initial trapped volume of liposomes and pe is an equivalent protein concentration at which molar concentrations of pores and liposomes become equal. Experimentally determined equivalent protein concentration pe is the basis of the method.

[A method of determining the specific volume of liposomes with Turnbull blue].

A method for measuring the specific volume of liposomes by using ferricyanide as a maker is proposed. Ferricyanide is determined by the formation of Turnbull's blue. Advantages of the use of this reaction are an eightfold increase in the sensitivity and the possibility to determine the marker compound in the spectral region (730 nm) free of any considerable interference of natural compounds.

Formation of taste aversion and preference in protein synthesis inhibition in rats.

The investigation was devoted to the role of the synthesis of protein and peptide factors during the formation of chemosensory memory in rats. Two models of gustatory memorization were used: conditioned taste aversion (CTA), induced by the association of the taste of saccharine with a toxic injection of lithium chloride, and enhanced taste preference (ETP), induced by the influence of preliminary drinking of a saccharine solution on its repeat consumption. It was found that, under conditions of the inhibition of protein synthesis in the brain of 43% by cycloheximide and of 59% by 8-azaguanine, CTA does not form.

[The formation of taste aversion and preference under conditions of protein synthesis inhibition in rats].

The study is devoted to the role of protein synthesis in formation of chemosensory memory in rats. Two experimental models of taste memorizing were used, i.e.

[Characteristics of the interaction between 2-alkylmalonates and the substrate-binding site of the dicarboxylate carrier of rat liver mitochondria].

The effect of thirteen synthetic 2-n-alkyl derivatives of malonic acid on the rate of the mitochondrial succinate oxidase reaction controlled by the dicarboxylate carrier, was studied. These compounds were shown to act as competitive inhibitors of succinate transport and could interact with the carrier according to the 1:1 stoichiometry. The affinity of the inhibitor for the carrier increased in parallel with the increase in the alkyl residue length.

[The health status and the indices of urinary system activity in children with long-term exposure to drinking water with an adverse mineral content].

A study was made of the effect of drinking highly mineralized water on the children's health status, particularly on the urinary system activity. As many as 357 children living in one of the poorly irrigated regions of the Saratov land along the Volga were examined. It has been established that drinking highly mineralized water is a high-intensity factor producing an untoward effect on the children's physical development, contributing to the rise of the incidence of acute (mainly respiratory) and chronic diseases (cholecystopathies, nephropathies, gastrointestinal diseases, nocturnal enuresis, dental caries).