Publications by authors named "Lagier J"
Over the past 12 years, culturomics, a high-throughput culture method, has been developed, considerably widening the repertoire of known cultured bacteria. An exhaustive database, including a list of microbes isolated by culture from human skin, was recently established by performing a review of the literature. The aim of the present study was to use the culturomics approach to explore the African skin microbiota.
View Article and Find Full Text PDF: The aim of the study was to compare dacryocystectomy (DCT) versus dacryocystorhinostomy (DCR) in patients with dacryocystitis in terms of tearing complaints. : We conducted a retrospective and comparative study on 19 patients. The main outcome measure was defined as an improvement by 1 point of the Munk score postoperatively.
View Article and Find Full Text PDFObjectives: Catheter removal is recommended in adults with Staphylococcus aureus central-line-associated bloodstream infection (CLABSI) but is controversial in children with long-term central venous catheters (LTCVC). We evaluated the occurrence of catheter salvage strategy (CSS) in children with S. aureus LTCVC-associated CLABSI and assessed determinants of CSS failure.
View Article and Find Full Text PDFIs topical antibiotic use necessary to prevent surgical site infection following oculoplastic surgery?
Graefes Arch Clin Exp Ophthalmol
October 2024
Purpose: To assess whether oculoplastic surgeries can be performed without any topical and systemic antibiotics, in a "100% antibiotic free" fashion.
Method: We conducted a multicenter retrospective study between November 2017 and December 2022. Patients who underwent an oculoplastic procedure were screened.
Article Synopsis
- The study assessed the asymptomatic prevalence of respiratory pathogens in a rural area of Senegal, focusing on individuals not showing symptoms of illness before the COVID-19 pandemic.
- A total of 368 nasopharyngeal swabs were collected from 15 participants over a year, with findings revealing 19.56% of samples positive for viruses and 13.60% for bacteria, notably during the rainy season when pathogen carriage increased.
- The results indicate significant circulation of respiratory viruses and bacteria in the community, suggesting a higher prevalence of asymptomatic carriers than previously recognized.
The emergence of the coronavirus pandemic facilitated the acquisition of mutations in the SARS-CoV-2 genome, resulting in the appearance of new variants over the past three years. We previously identified several taxa associated with the clinical outcome of COVID-19 disease in a retrospective study involving 120 patients (infected patients and negative subjects). However, little is known about whether the different variants could influence variations in the composition of the nasopharyngeal microbiota.
View Article and Find Full Text PDFArticle Synopsis
- Respiratory infections, primarily caused by viruses, are a major health issue globally, and a study was conducted in rural Senegal to investigate the presence of viruses and bacteria in symptomatic and asymptomatic individuals.
- The research found that 83.6% of symptomatic individuals tested positive for at least one respiratory virus, with influenza A, metapneumovirus, and enterovirus being notably more common among those with symptoms, while 21.8% of asymptomatic individuals also had viral infections.
- Additionally, 82.0% of symptomatic subjects carried at least one respiratory bacterium, with Moraxella catarrhalis and Streptococcus pneumoniae being the most common, highlighting the need for further surveillance of these pathogens
Background And Aims: Gut microbial imbalances are linked to colorectal cancer (CRC), but archaea's role remains underexplored. Here, using previously published metagenomic data from different populations including Austria, Germany, Italy, Japan, China, and India, we performed bioinformatic and statistical analysis to identify archaeal taxonomic and functional signatures related to CRC.
Methods: We analyzed published fecal metagenomic data from 390 subjects, comparing the archaeomes of CRC and healthy individuals.
During the current global outbreak of mpox (formerly monkeypox), atypical features were frequently described outside endemic areas, raising concerns around differential diagnosis. In this study, we included 372 adult patients who had clinical signs consistent with mpox and who were screened using non-variola orthopoxvirus specific quantitative polymerase chain reaction (PCR) between 15 May and 15 November 2022 at the University Hospital Institute Méditerranée Infection, Marseille, France. At least one clinical sample was positive for 143 (38.
View Article and Find Full Text PDFColorectal Cancer Archaeome: A Metagenomic Exploration, Tunisia.
Curr Issues Mol Biol
September 2023
Colorectal cancer (CRC) is a serious public health problem known to have a multifactorial etiology. The association between gut microbiota and CRC has been widely studied; however, the link between archaea and CRC has not been sufficiently studied. To investigate the involvement of archaea in colorectal carcinogenesis, we performed a metagenomic analysis of 68 formalin-embedded paraffin fixed tissues from tumoral ( = 33) and healthy mucosa ( = 35) collected from 35 CRC Tunisian patients.
View Article and Find Full Text PDFPreliminary landscape of Saccharibacteria in the human microbiome.
Front Cell Infect Microbiol
August 2023
Article Synopsis
- * Research methods included both prospective and retrospective screening techniques, such as PCR, real-time PCR, and scanning electron microscopy, to analyze the prevalence of TM7 in different human samples.
- * Findings revealed that TM7 were most common in oral samples, with lower prevalence in other bodily fluids, and even found in some infectious samples, highlighting their status as human commensals and suggesting the need for more research on their potential role in disease contexts.
Blood is a precious biological liquid that is normally sterile. Therefore, bacteria in the bloodstream are shown a priori anomaly. A blood culture is systematically performed to diagnose the cause of the bacteremia.
View Article and Find Full Text PDFBackground: The safety and efficacy of botulinum neurotoxin type A (BoNTA) treatments are well established, but injection techniques, target muscles, and toxin doses continue to evolve, with each refinement producing improvements in treatment outcomes. The recommendations in this consensus move away from standard templates and illustrate how to tailor treatments to individual patterns and strengths of muscle activity, and patient preferences.
Methods: Seventeen experts in the fields of plastic surgery, dermatology, ophthalmology, otorhinolaryngology, and neurology convened in 2022 to develop consensus-based recommendations for the use of botulinum toxin A for the treatment of horizontal forehead lines, glabellar frown lines, and crow's feet lines that reflect current clinical practice.
Article Synopsis
- A significant outbreak of Monkeypox virus (MPXV) was reported in nonendemic countries starting in May 2022, leading to a study of clinical samples from infected patients between June and July 2022.
- Using advanced DNA metagenomics and sequencing technologies, researchers classified the genomes of MPXV from 18 patients, finding a high number of mutations compared to previous strains, specifically in clade IIb, lineage B.1.
- The study highlighted the role of specific mutations in crucial viral genes and recommended enhanced genomic and clinical monitoring of MPXV and related bacterial infections in patients to better understand the virus's evolution and infection complications.