Publications by authors named "Lady Giovanna Ramirez"
Article Synopsis
- - Neutrophils play a crucial role in fighting infections, but their function in drug-resistant infections, like leishmaniasis, is not well understood.
- - Research shows that drug-resistant parasites change the expression of certain neutrophil genes, increasing detoxification processes and reducing cytokine production, particularly impacting a chemokine called CCL3.
- - Reduced CCL3 levels hinder neutrophils’ ability to recruit other immune cells, which was confirmed in mouse models, highlighting how drug-resistant parasites can alter immune responses in infected skin.
Obtaining high quality RNA from skin biopsies is complex due the physical composition and high content of nucleases of this tissue. This becomes particularly challenging when using compromised skin samples with necrotic, inflammed or damaged areas, such as those from patients suffering skin conditions, which affect more than 900 million people annually. We evaluated the impact of the biopsy size and tissue preservation method on the quality and quantity of RNA extracts.
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- Recent research shows that the body's natural immune response may play a role in how well antimicrobial drugs work, particularly against leishmaniasis.
- The study focused on how clinical strains of parasites resistant to a common antileishmanial drug, pentavalent antimony (SbV), affect neutrophil activation by comparing sensitive and resistant strains.
- The findings indicate that neutrophils infected with drug-resistant strains produce less reactive oxygen species and have a different expression profile for specific activation markers compared to those infected with drug-sensitive strains.