Microbiota entrapped in recently-formed ice: Paradana Ice Cave, Slovenia.

Paradana is one of the biggest ice caves in Slovenia, with an estimated ice volume of 8,000 m. Reflecting climatological conditions, the cave ice undergoes repeated freeze-thaw cycles and regular yearly deposition of fresh ice. Three distinct ice block samples, collected from the frozen lake in May 2016, were analysed to obtain data on ice physicochemical properties and the composition of associated microbiota.

Syringe life and memory effects in isotopic analyses performed by liquid water isotopic analysers - a case study for natural waters from central Europe.

Syringes are the most expensive consumables for analyses in the Liquid Water Isotope Analyser using commercial Cavity Ring-Down Spectroscopy (CRDS) systems. Our experience shows that although the syringe life significantly varies, it is not an exception that a syringe performs many thousands of injections. A simple maintenance procedure consisting of replacing the syringes two times per day and keeping them in the deionised water resulted in an average syringe life of over 3500 injections.