Immunochemical identification of ubiquitin and heat-shock proteins in corpora amylacea from normal aged and Alzheimer's disease brains.

Corpora amylacea (CA) accumulation in the central nervous system (CNS) is associated with both normal aging and neurodegenerative conditions such as Alzheimer's disease (AD). CA is reported to be primarily composed of glucose polymers, but approximately 4% of the total weight of CA is consistently composed of protein. CA protein resolved on sodium dodecylsulfate-polyacrylamide gel electrophoresis showed a broad range of polypeptides ranging from 24 to 133 kDa, with four abundant bands.

Ubiquitin is a component of polypeptides purified from corpora amylacea of aged human brain.

Corpora amylacea (CA) are one of the conspicuous features of brain tissue in normal aging and neurodegenerative diseases. Quantitative protein determination of purified CA revealed a protein content of about 4% of total weight. Qualitative protein analysis revealed a broad range of polypeptides, with four being more abundant.

Lack of association between two restriction fragment length polymorphisms in the genes for the light and heavy neurofilament proteins and Alzheimer's disease.

The etiology of Alzheimer disease (AD) remains unknown. The hypothesis of genetic factors playing a role in the causation of the disease, at least in its familial form, has been borne out by results showing linkage in several early-onset AD families to a locus on the proximal part of the long arm of chromosome 21. Linkage was not detected in several other families using the same markers.

Purification and polypeptide composition of corpora amylacea from aged human brain.

Corpora amylacea (CA) accumulation in the brain is a normal correlate of ageing. The presence of a small amount of protein in these polyglucosan bodies is a consistent finding, although the nature of this protein material remains unknown. Using sucrose gradient fractionation and density centrifugation on Percoll, a method was developed to obtain highly pure preparations of CA from human brain.

Ascertainment of informative Alzheimer disease families from the IMAGE Project registry for genetic linkage analysis studies.

Genetic linkage analysis requires the identification and documentation of large families with many affected members present, preferably in more than one generation. The IMAGE Project has been establishing a population-based Alzheimer disease (AD) registry in the Saguenay - Lac-Saint-Jean region of the Province of Quebec. The population of this region has a well-documented ancestry, with reliable genealogical records (since 1842) computerized by SOREP.

Immunocytochemical Localization of Ribulose-1,5-Bisphosphate Carboxylase in the Pyrenoid and Thylakoid Region of the Chloroplast of Chlamydomonas reinhardtii.

The distribution of the large and small subunits of ribulose-1,5-bisphosphate carboxylase in the chloroplast of Chlamydomonas reinhardtii was studied by immunoelectron microscopy by labeling Lowicryl-embedded sections with antibody to each subunit followed by protein A-gold. In light-harvested synchronously dividing cells, antibodies to each subunit heavily labeled the pyrenoid, whereas the thylakoid region of the plastid was lightly labeled. By estimating the volume of each chloroplast compartment, it was determined that approximately 40% of the total small subunit in the plastid and 30% of the large subunit are localized in the thylakoid region, presumably in the stroma.

Ochromonas mitochondria contain a specific chloroplast protein.

Antibody raised against the small subunit of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.

Distribution of U3 snRNA in the nucleolus of CHO cells.

Localization of snRNA at the ultrastructural level was studied in the nucleolus of CHO cells by EM autoradiography. In conditions where snRNA U3 is the only RNA species labelled in the nucleolus, silver grains were largely found at the periphery, over the granular ribonucleoprotein component and the perinucleolar condensed chromatin; this enrichment was quantitatively significant. Inhibition of pre-rRNA synthesis with actinomycin D did not alter the concentration or the distribution of U3 inside the nucleolus.

Localization of small nuclear RNA by EM autoradiography in Chinese hamster ovary (CHO) cells.

The ultrastructural localization of small nuclear RNA (snRNA) was studied by EM autoradiography in Chinese Hamster Ovary (CHO) cells. Conditions were set where most (greater than 85%) of the nuclear [3H]uridine label consisted of snRNA, the most abundant species being U1, U2 and the nucleolar species U3. The label was found in highest density in the peripheral part of the nucleus, especially over areas of condensed chromatin.