4-acetamido-3,3,5,5-[2H4]-2,2,6,6-tetra([2H3]methyl)piperidin-1-yloxyl 0.33-hydrate.

The asymmetric unit of the title compound, C11H5D16N2O(2).0.33H2O, is formed by three crystallographically independent piperidin-1-yloxyl molecules and a molecule of water.

Hydrophilic polymers--biocompatibility testing in vitro.

Biocompatibility is one of the main prerequisites for safe use of medical devices. Estimation of cytotoxicity is a part of the initial evaluation laid down in ISO standards on biological evaluation of medical devices. Hydrophilic polymers (based on 2-hydroxyethyl methacrylate HEMA) doped by addition of selected additives with antioxidant and/or free radical scavenging potential (vitamin C and hindered amine stabilizer N-(2,2,6,6-tetramethylpiperidin-4-yl)methacrylamide) were tested in different in vitro systems (3T3 Balb/c cell culture and a 3D human skin model) for biocompatibility and suitability for use as wound dressings.

Binding of D-mannose to poly(2-hydroxyethyl methacrylate) hydrogels by azo coupling.

Crosslinked hydrogels based on poly(2-hydroxyethyl methacrylate) poly(HEMA) were prepared and modified with tyramine [4-(2-aminoethyl)phenol] to enable azo coupling with mannosyl derivatives, copolymerization of HEMA with polymerizable activated esters and surface modification reaction with 4-nitrophenyl chlorocarbonate for activation of the hydrogels were used.

Mannosides as crucial part of bioactive supports for cultivation of human epidermal keratinocytes without feeder cells.

Large-scale production of keratinocytes by cell culture is of interest for medical applications. Long-term cultivation of epidermal cells is presently possible with feeder cells, i.e.

[The histopathologic picture of experimental poisoning with ethylmethacrylates].

Toxic effect of hydroxyethylmethacrylate, acetoxyethylmethacrylate and diethylenglycomethacrylate were studied in rats surviving as long as 1 to 20 days after intramuscular administration. Conspicuous lesion were found only in calf muscles at the site of application. Muscle fibre necroses with inflammatory reaction occurred repeatedly in animals surviving 1-2 days.

Reactive oxygen species (ROS) generated by xanthine oxidase in the corneal epithelium and their potential participation in the damage of the corneal epithelium after prolonged use of contact lenses in rabbits.

Prolonged use of contact lenses (for 14 days) evoked an imbalance between the activity of xanthine oxidase (an enzyme belonging to reactive oxygen species-generating oxidases) and catalase (an enzyme belonging to reactive oxygen species-scavenging oxidases) in the corneal epithelium of rabbits. The activity of catalase decreased, while xanthine oxidase activity was very high. Of other enzymes studied in the corneal epithelium, the activities of xanthine oxidoreductase, glucoso-6-phosphate dehydrogenase and succinate dehydrogenase were decreased.

[New approaches in clinical ophthalmology: UV absorbers and hindered amines covalently bound to contact lenses. Importance of enzyme histochemistry in studying their effectiveness in vivo].

UV absorbers developed by us are covalently bound in hydrophilic contact lenses. This is great advantage because UV absorbers cannot be diluted from contact lenses. In laboratory investigations and "in vivo" experiments it was found that contact lenses containing UV absorbents prevent the eye against the damaging effect of UV irradiation (UVA, UVB, UVC rays).

[Distribution of 3H-labeled acetyl-HEMA monomer in rats].

Original method of tritiation of acetyl-HEMA and its hydrogenated product was developed. Distribution and excretion of both compounds was examined in rat for up to 70 hrs. While the distribution in the tissues examined (liver, spleen, kidney, lung, muscle, heart, and skin) was nearly uniform, the excretion of acetyl-HEMA and its hydrogenated form differed: acetyl-HEMA in the 24.

Toxicity of monomers HEMA, DEGMA and AEMA, used in synthesis of hydrogels for medical applications.

Acute toxicity parameters (lethal doses) were evaluated and calculated after i.m. and i.

Disposition of lypophilized (methylmethacrylate-14C, 2-hydroxyethylmethacrylate, butylacrylate) nanoparticles in rats and their effect on zoxazolamine paralysis time.

The fate of lyophilized (methylmethacrylate-14C, 2-hydroxyethylmethacrylate, butylacrylate) nanoparticles was studied in male Wistar rats after p.o. administration.

Fate of 14C-terpolymer (methylmethacrylate-14C, 2-hydroxyethylmethacrylate, butylacrylate) nanoparticles after peroral administration to rats.

The fate of 14C-terpolymer (methylmethacrylate-14C, 2-hydroxyethylmethacrylate, butylacrylate) nanoparticles was studied in male Wistar rats after peroral administration. These nanoparticles may reach systemic circulation as evidenced by the plasma 14C level, excretion of the label in the urine, as well as organ label deposition. It was found that at least 2% of the dose of 14C was absorbed from the gastrointestinal tract.

Determination of the interaction of lactate dehydrogenase with high-molecular-weight derivatives of AMP by affinity electrophoresis.

The interaction of lactate dehydrogenase with high-molecular-weight derivatives of AMP was studied by affinity electrophoresis in an alkaline buffer system and by means of kinetic measurements. AMP was coupled to synthetic hydroxypropylmethacrylamide copolymers through glycine, 6-aminohexanoic and 12-aminododecanoic spacer arms. The values of the dissociation constants (K) of the lactate dehydrogenase isoenzymes--immobilized AMP complexes determined by affinity electrophoresis decreased with increasing length of the spacer arm.

Reactive carriers of immobilized compounds.

Sphericanl macroporous reactive carriers capable of forming covalent bonds with amino acids and proteins were prepared by the suspension copolymerization of 2-hydroxyethyl methacrylate, ethylene dimethacrylate and p-nitrophenyl esters of methacrylic acid and methacryloyl derivatives of glycine, beta-alanine and epsilon-aminocaproic acid. The effect of the spacer length, pH and the type of the buffer used, concentration of reactive groups in the copolymer, concentration of the ligand and the participation of the hydrolytic and aminolytic reaction of p-nitrophenyl functional groups in the attachment of glycine, D,L-phenylalanine and serumalbumin was studied. Macroporous copolymers containing reactive functional groups can be used as active enzyme carriers, if their activity is not blocked by the presence of p-nitrophenol split off in the attachment reaction.