[NON-CONVULSIVE EPILEPTIC SEIZURE IN PATIENT WITH DELAYED AWAKENING AFTER SUPRATENTORIAL TUMOR REMOVAL].

In some cases after elective neurosurgical procedures we use technique of delayed awakening of patients. Prolonged sedation however can hide the development of epileptic seizures and lead to the status epilepticus formation. This article is a demonstration and discussion of a clinical case of nonconvulsive status epilepticus during delayed awakening of the patient due to prolonged sedation after elective neurosurgical interventionsforsupratentorial brain tumor Presented case suggests the presence of significant risk of nonconvulsive status epilepticus during prolonged sedation.

[Effect of carbohydrates and polyols on amide content and protein fragmentation in a lactoglobulin preparation].

Certain carbohydrates and polyols are used at various stages of the production of immunobiological preparations as stabilizers of biological activity, particularly in the production of lactoglobulin (against opportunistic pathogens) using membrane ultrafiltration. This study concerns the effect of these substances on changes in the amide content in proteins of this lactoglobulin. Lactoglobulin was incubated in near-physiological (0.

The effect of hydrocarbon addition on the deamidation rate in immune whey proteins.

The influence of some hydrocarbons that are often used at different stages of immunobiological preparation's production as stabilizers of biological activity on the dynamics of nonenzymatic deamidation in proteins of immune whey against conditionally pathogenic microorganisms obtained by means of membrane ultrafiltration technology is investigated. Preparations of whey were incubated in 10 per cent solutions of glucose, fructose and sorbitol at the conditions similar to physiological ones (0.9% NaCl, pH 5.

[Free radical processes and antioxidant system in depression and treatment efficiency].

Free radical processes and antioxidant systems activities were studied before and after treatment in blood and saliva of 23 depressive patients with ICD-10 diagnosis--6 with depressive episode and 17 with recurrent depression. Compared to control, blood plasma Fe(2+)-rodamine induced chemiluminescence, total peroxidase and catalase activities increased and blood plasma superoxide eliminational activity as well as superoxide dismutase activity in erythrocytes decreased in patients before treatment. At the same time, total peroxidase activity, superoxide eliminational activity and catalase activity were elevated in the patient's saliva.

[Intensity of free radical process and antioxidant enzymes activity in human saliva and plasma under emotional stress].

The intensity of free radical processes (FRP) and the activity of antioxidant (AO) systems in human blood plasma and saliva were studied under physiological emotional strain and depressive pathologies (before and after treatment). The significant alterations in the dynamics of investigative indexes under different emotional strain were revealed. These alterations in saliva were more intensive than in blood plasma.

[Dynamics of luminol-dependent chemiluminescence in rat whole blood at different oxygen concentrations].

Chemiluminescence analysis was used to examine the yeast cell-activation of rat whole blood neutrophilic leukocytes in rats after their pre-exposure to 3-hour hypoxia (9,000 m above sea level) and two-hour hyperoxia (0.3 MPa), as well as their resultant effects. The hypoxia was found to cause a significant (42%) reduction in the length of the latent period, suggesting that it activates the phagocytic activity of neutrophilic leukocytes.

[Chemiluminescent analysis and some indicators of nitrogen catabolism in rat blood plasma in hypoxia with subsequent hyperoxia].

Different regimens of oxygenation were studied in rats: acute hypoxia (9,000 m, 3 hrs), acute hyperoxia (0.7 MPa O2), which caused convulsions, and their simultaneous effects. Under these conditions the following parameters were evaluated: the rate of Fe(2+)-induced chemiluminescence, content of nitrogen and peptide catabolism products (urea, urates and middle molecule peptides) as well as total peroxidase activity, content of extraerythrocyte hemoglobin and lactic acid in blood plasma.

[The effect of pyrazidole on the middle molecule level during various modes of hypobaric hypoxia].

Content of middle mass molecules (MMM) was studied in blood plasma of rats treated with pyrazidol, the known inhibitor of monoamine oxidase of the A type. A distinct increase in the MMM nonpeptide component was detected both in intact animals and in the rats kept under hypoxic conditions (9,000 m, 3 hrs) after three administrations of pyrazidol. Under conditions of intermittent hypoxia higher increase of the nonpeptide component as well as an increase of the MMM peptide component were detected.

[Emoxipin correction of disorders of lipid peroxidation as affected by a slight excess of oxygen pressure].

The role of the emoxipin (Em.) (2-ethyl-6-methyl-3-oxipyridine) in the correction of the free radical oxidation and allied processes in lung tissues and blood plasma under high-pressure oxygen-prolonged action has been investigated. The studied oxygen exposure (0.

[Micro-determination of the intensity of luminol-dependent chemiluminescence of plasma].

The authors attempted to modify the macro-method for assessment of the intensity of luminol-dependent chemiluminescence by using small volumes of capillary blood, diluted 100-fold. They have demonstrated the compatibility of two methods for blood collection, from the vein and from the finger, on the basis of the rapid test of oxygen intoxication severity and individual sensitivity of the body to hyperoxia in vitro. Use of microvolumes of the sample for analysis will facilitate the clinical application of the test for the selection of the optimal HBO schemes.

[Changes of uric acid levels in rat tissues as a systemic reaction to hyperoxia].

The levels of tissue antioxidant uric acid in relation to chromosomal aberrations and leaving erythrocytic chromatin were determined in the spleen, bone marrow and blood of rats exposed to toxic hyperoxia (0.7 MPa O2, convulsions). Considerable growth of the uric acid levels and the rate of chromosomal aberrations in all tissues was observed within the first hours after treatment.

[Biochemical indicators of oxygen poisoning].

Content of extra erythrocyte hemoglobin (EEH), total peroxidase activity (TPA), tseruloplasmin oxidase activity (TOA) and chemiluminescence intensity have been investigated in plasma. The dose-dependent changes of EEN, TPA, TOA are shown in experiments on the animals under hyperbaric oxygen (HBO) pressure (0.2, 0.

[Iron-containing proteins in the plasma and serum of the blood in patients during hyperbaric oxygenation therapy].

Extra-erythrocyte hemoglobin (EEH) level and total peroxidase activity (TPA) have been analysed in the blood serum and plasma of 44 patients following hyperbaric oxygenation (HBO). The levels of the parameters tested decreased after 3 HBO procedures and remained low to the end of the treatment period in the first group of patients. In the second group of patients, on the contrary, EEH and TPA levels increased after 3 HBO procedures and remained high to the end of the treatment period.

[Changes in the proteolytic activity of tissues during aging].

The intensity of proteolytic processes and qualitative composition of autolysis products of the brain, liver and testicle tissues of young and old rats were studied. The gel-chromatographic analysis (Sephadex G-15 and G-50) revealed no considerable amount of high-molecular peptides (1500 Da and over) before and after autolysis. The measurement of the quantity of free amino groups in the gel-chromatographic fraction after the complete acid hydrolysis has confirmed that result.

[Use of blood biochemical parameters for evaluating the clinical effectiveness of hyperbaric oxygenation therapy].

Changes in the total peroxidase activity (TPA) and extraerythrocyte hemoglobin (EEH) content have been studied in the blood plasma and serum of patients treated with hyperbaric oxygenation (HBO). TPA and EEH content after the 3rd HBO session increased by 38% and 257%, respectively, in the plasma and by 56% and 74%, respectively, in the serum. By the end of HBO therapy course, after the 8th session, plasma level of the parameters under study normalized, while their serum level was higher than baseline.

[Amidation of blood proteins during hyperglycemia in experimental diabetes mellitus].

Rates of amidation of blood serum albumin and hemoglobin was studied in hyperglycemia developed during alloxan diabetes mellitus. Content of glucose in blood of the animals with diabetes exceeded 5.2-fold its level in blood of controls; amount of glycosylated hemoglobin was increased by 41.

[Effect of starvation on the intensity of proteolysis and amidation of proteins in tissues of rats of different ages].

Rates of autolysis and degree of amidation of tissue proteins were studied in rats under conditions of ageing and after starvation during 7 days. Proteins with decreased content of amide groups accumulated in brain, liver tissues and skeletal muscles. This phenomenon occurred apparently due to decrease in the rate of protein autolysis observed in ageing.

[Changes in the fluorescence in preparations of normal human immunoglobulin during deamidation].

It was shown that self-fluorescence of normal human immunoglobulins prepared with the use of donor and placental raw materials lowered after incubation at 37 degrees C for 28 days and after storage under the standard conditions (4-10 degrees C) for 2 years. Dependence of thermostability of the immunoglobulins on the raw material used for their preparation and the storage period was revealed spectrofluorometrically. Resistance of the proteins to thermal denaturation lowered on storage.

[The effect of various chemical compounds and storage conditions on the rate of non-enzymatic deamidation of protein preparations].

The effect of different substances partly used as preservatives for the blood storage and at different stages of manufacturing of human blood preparations on the dynamics of nonenzymatic deamidation of commercial protein preparations and on their heat stability was being studied. Albumin and gamma-globulin preparations in the solutions of 60% glycerol, 60% ethylene glycol, 40% beta-alanine, aspartic and glutamic acids in physiological concentrations, 40% glucose and 40% sucrose after 2-hour thermal denaturation (100 degrees) were incubated under (or close to) physiological conditions (pH 7, 37 degrees) for 0.7; 14, 21, 28 and 90 days.

[Effect of non-enzymatic desamidation of gamma-globulin on its properties and biological activity].

During incubation of gamma-globulin preparations under conditions similar to physiological ones within 28 days (0.9% NaCl, pH 7.0, 37 degrees) as well as during storage at 3-10 degrees within 36 months gradual nonenzymatic deamidation of the protein occurred mainly due to hydrolysis of readily accessible amide groups (asparagine).

[Degradation of deamidated proteins by tissue proteinases].

The rate of native and deamidized serum albumin in vitro splitting by the brain, liver, kidney, testicle and spleen tissue proteinases was studied at pH 3.2, 4.8, 7.

[Biochemical estimation of individual sensitivity to oxygen intoxication in rabbits].

Correlation between a pro-oxidant activity of blood, estimated by means of chemoluminescence in the system H2O2-luminol-blood plasma, and individual sensitivity of rabbits to the effect of oxygen was studied. Alterations in the blood pro-oxidant activity, as shown by treatment of the blood sample with 0.7 MPa of oxygen in vitro, correlated distinctly with the period of convulsions as well as with viability of animals during acute hyperoxia.