Multisite evaluation of a monoclonal IMMULITE erythropoietin immunoassay.

Background: Erythropoietin (EPO) measurements are useful in diagnosing anemias and polycythemias. We conducted a multisite evaluation of a monoclonal IMMULITE® EPO immunoassay.(1) DESIGN AND METHODS: The IMMULITE EPO assay is a solid-phase enzyme-labeled chemiluminescent immunometric assay.

A weak cation-exchange monolith as stationary phase for the separation of peptide diastereomers by CEC.

A CEC weak cation-exchange monolith has been prepared by in situ polymerization of acrylamide, methylenebisacrylamide and 4-acrylamidobutyric acid in a decanol-dimethylsulfoxide mixture as porogen. The columns were evaluated by SEM and characterized with regard to the separation of diastereomers and α/β-isomers of aspartyl peptides. Column preparation was reproducible as evidenced by comparison of the analyte retention times of several columns prepared simultaneously.

Capillary electrophoresis-based sirtuin assay using non-peptide substrates.

Sirtuins are NAD(+)-dependent class III histone deacetylases, which catalyze the deacetylation of acetyl-lysine residues of histones and other protein substrates yielding the deacetylated protein, nicotinamide and 2'-O-acetyl-ADP-ribose. Two lysine amide derivatives containing dansyl (Dns) or 7-dimethylaminocoumarin (DMAC) residues, i.e.

Separation of peptide diastereomers using CEC and a hydrophobic monolithic column.

A neutral hydrophobic monolith prepared by radical in situ copolymerization of lauryl methacrylate and ethylene dimethacylate has been evaluated for the CEC separation of diastereomers of small peptides using acidic mobile phases containing ACN as organic modifier. Using an acidic mobile phase, the peptides migrated due to their own electrophoretic mobility. Hydrophobic interactions with the stationary phase contributed to the separation.

Application of phase optimized liquid chromatography to oligopeptide separations.

Stationary phase optimized liquid chromatography (POPLC) has been applied to the separation of oligopeptides. The retention factors and theoretical plate numbers of 13 peptides were determined on five different stationary phases. Based on these values, an optimal stationary phase composition of 250mm total length consisting of 3 segments of 20mm octadecyl silica, 10mm phenyl silica and 220mm embedded polar octadecyl silica was calculated by the optimizer software.

9-Fluorenylmethoxycarbonyl-labeled peptides as substrates in a capillary electrophoresis-based assay for sirtuin enzymes.

Sirtuins are the class III histone deacetylases that catalyze the deacetylation of acetyl-lysine residues of histones and other proteins using nicotinamide adenine dinucleotide (NAD(+)) as the cofactor. The reaction yields the deacetylated protein, nicotinamide, and 2'-O-acetyl-ADP-ribose. Three 9-fluorenylmethoxycarbonyl (Fmoc)-labeled peptides derived from the amino acid sequence of p53, Fmoc-KK(Ac)-NH(2), Fmoc-KK(Ac)L-NH(2), and Fmoc-RHKK(Ac)-NH(2), were characterized as substrates for two of the human sirtuins: SIRT1 and SIRT2.

Development of a capillary electrophoresis-based assay of sirtuin enzymes.

Sirtuins are a family of nicotinamide adenine dinucleotide (NAD(+))-dependent enzymes catalyzing the deacetylation of acetyl-lysine residues of histones and other proteins. Three 9-fluorenylmethoxycarbonyl (Fmoc)-labeled peptide substrates derived from the amino acid sequence of p53, i.e.

[Possibilities and limits of medical graphology. Determination of current status and perspectives (I)].

As is generally known, the writing movement reacts in a sensitive manner to numerous diseases, medications, stimulants and environmental poisons, as well as to psychic and physical influences. This reaction can occur quite early in the process. Therefore, laboratory data and the physical examination can be supplemented by the handwriting.

[The controversy regarding the cause of death of Wolfgang Amadeus Mozart (28 January 1756-5 May 1791)].

All since two hundred years closely contested hypotheses about the causes of Wolfgang Amadeus Mozart's death are to be verified and to be made credible by the same "original sources" in spite of considerable contrasts. In the present study it is tried to indicate the reasons for the multifariousness of the opinions and to represent Mozart's death as the consequence of a multifactorial process.

[Possibilities and limitations of rapid radiologic detection of selected chemicals in acute poisoning of unknown origin].

Knowledge of the radiopacity of bromine-containing hypnotics and of halogenated hydrocarbons has led to numerous recommendations and speculations. Supported by own investigations the sensible application of a rapid radiological determination in the practice of clinical toxicology is discussed. This should always be carried out, if solvent residues, gastric contents or gastric lavage fluid are available, a ingestion is suspected, and the result of chemical analysis is not promptly obtainable.

[Etiologic and pathogenetic aspects of the demyelinating process in multiple sclerosis].

In our opinion there is no specific germ for multiple sclerosis. The pathogenic process is developed after a clinically "silent" period and results in a demyelinization process. Partially genetical aspects have to take into account for certain disease processes.

[Progress of research in the diagnosis of multiple sclerosis by study of the cerebrospinal fluid].

There are neither specific cell investigations nor immunological tests for the diagnosis of MS. Various immunological alterations occur in the course of the disease, but none is unique to MS. Accurate diagnosis remains confined to the clinical field.

[Clinical and pathophysiologic aspects of viral infections of the CNS].

The available defensive mechanisms are generally sufficient to protect the central nervous system (CNS) against the penetration of infectious particles. The blood-brain-barrier plays a decisive role in this process. The present paper represents the most important circumstances realizing that: 1.