Seasonal variations in the harpagoside content of Scrophularia scorodonia L.

Seasonal variations on the content of harpagoside in Scrophularia scorodonia L. (Scrophulariaceae) were investigated using plants collected monthly from January to December in 1995. During growth of this species the percentage of harpagoside was the highest during the maximum development of the plant, specially in July.

A sulfated saponin from bupleurum rigidum.

A new sulfated triterpene glycoside with the sulfate group located in an unusual position in the carbohydrate moiety, was isolated from the MeOH extract of the aerial parts of Bupleurum rigidum. This compound was identified by a combination of chemical degradation and spectral methods as 3beta,16beta,23-trihydroxy-13, 28-epoxyolean-11-en-3beta-yl-beta-D-glucopyranosyl-(1-->2)[4-sulfate- beta-D-glucopyranosyl-(1-->3)]-beta-D-fucopyranoside (sandrosaponin I) (1). In addition, the known compound 3beta,16beta, 23-trihydroxy-13, 28-epoxyolean-11-en-3beta-yl-beta-D-glucopyranosyl-(1-->2)[beta-D-glu copyranosyl-(1-->3)]beta-D-fucopyranoside (2) was isolated in the present investigation.