Modeling of 3D Structure of Chimeric Constructs Based on Hemagglutinin of Influenza Virus and Immunogenic Epitopes of Streptococcus Agalactiae.

A project of an experimental recombinant vector vaccine for prevention of diseases caused by pathogenic streptococci based on ScaAB lipoprotein of Streptococcus agalactiae and a coldadapted strain of live influenza vaccine as a vector was developed. The sequence of ScaAB lipoprotein was analyzed and fragments forming immunodominant epitopes were determined. Chimeric molecules of influenza virus hemagglutinin H7 carrying insertions of bacterial origin were constructed.

[Intranasal co-administration of recombinant streptococcus group B polypeptides and influenza deltaNS1 vaccine].

In the present work, the immunoadjuvant properties of the influenza deltaNS1 vaccine virus after intranasal administration in combination with recombinant GBS polypeptides was tested in mice. According to our data, co-administration of recombinant GBS polypeptides and influenza deltaNS1 vaccine resulted in the increase in the immunogenicity and protective efficacy of bacterial proteins. Combined vaccination with the GBS polypeptides and influenza deltaNS1 vaccine has a potential to be used not only for prophylaxis infections caused by SGB, but also for prevention of the bacterial complications of influenza.

[Recombinant fragments of conservative proteins of group B streptococci as a basis of specific vaccine].

Aim: The study devoted to problem of using of recombinant fragments of group B streptococci (GBS) conservative proteins for induction of immune response against streptococcal infections. Two recombinant polypeptides (ScaAB and-ScpB1) corresponding to immunogenic epitopes of two surface GBS proteins ScaAB and C5a-peptidase, which are presented in other streptococcal species, were studied. The objective of the study was to assess specificity and protective activity of mentioned polypeptides against homologous and heterologous strains of pathogenic streptococci from different groups.

[Comparative study of experimental conjugated and combined vaccines against group B streptococci].

Immunogenic properties of recombinant protein ScaAB mixed with polypeptide P6 as well as conjugate of the former with capsular polysaccharide type III (PSIII) were assessed. Protein-polysaccharide conjugate was synthesized by reductive amination method. Immunogenicity was studied on the mouse model using aluminium hydroxide as adjuvant.

[Protective properties of certain external proteins of group B streptococci].

On the basis of genes, which control synthesis of externally localized proteins of group B streptococci (bac and scaAB), recombinant polypeptides P6 and ScaAB were obtained. Data on protective activity of these polypeptides during experimental infection of immunized mice as well as in opsonophagocytic test on cultivated peritoneal macrophages are presented. It has been shown that protective effect of specific antibodies to P6 was dependent from intensity of immune response.

[Immunogenicity of modified by dextran recombinant fragment of Bac protein of group B streptococci].

Opportunity to increase of immunogenicity of recombinant polypeptide P6 constructed on the basis of surface protective Bac protein by its chemical conjugation with dextran (D) 40 was studied. 3 preparations with different quantity of protein and polysaccharide components were obtained. Their testing with standard serum showed that antigenic determinants of the polypeptide were preserved although partly enclosed and structure of antigenic determinants did not significantly changed.

[Experimental evaluation of recombinant polypeptides as Streptococcus Group B vaccine].

Recombinant polypeptides corresponding to the conservative N-terminal area of Bac surface protein of group B streptococci (GBS) and having the human IgA binding-site were obtained and evaluated in terms of Immunogenic and protective properties. GBS strain 219, serotype 1 bc, served as the source of chromosomal DNA used for cloning. Three polypeptides P1, P5 and P6 were constructed.

Effects of Ca2+-ATPase inhibitors, ionomycin, and pharmacological modulators of ryanodine receptor on calcium release from intracellular pools and on oscillatory contractile behavior in Physarum polycephalum.

Changes in calcium levels in organelles of the plasmodium of the myxomycete Physarum polycephalum were analyzed using the fluorescent calcium indicator chlortetracycline (CTC). Both the Ca2+-ATPase inhibitor 2,5;-di(tert-butyl)-1,4-benzohydroquinone (BHQ) (100 microM) and the calcium ionophore ionomycin (1 microM) induce a significant decrease in fluorescence level (by 30%) in CTC-stained microplasmodia; this is caused by release of calcium from intracellular storage compartments. An activator of ryanodine receptors, caffeine (10-50 mM), is less effective on Ca2+ release than BHQ or ionomycin, and their inhibitor, ryanodine (100 microM), almost completely blocks the response to caffeine, but only slightly decreases the effects of BHQ or ionomycin.

[Polyclonal antiserum against noncatalytic part of cellobiohydrolase I from Trichoderma reesei].

A specific antiserum to the noncatalytic part of cellobiohydrolase I from Trichoderma reesei was obtained by exhaustion of rabbit antiserum to the native enzyme with its catalytic domain prepared by papain treatment of cellobiohydrolase I tightly adsorbed onto microcrystalline cellulose.

[The action of noradrenaline, vasopressin and deoxycorticosterone acetate on the activity of the Na+,K+ pump in the veins and arteries of different vascular regions].

Noradrenaline and vasopressin were shown to stimulate the Na+/K(+)-pump activity both in veins and in arteries, whereas desoxycorticosteronacetate did not increase it in pulmonary and mesenteric vessels and even depressed it in the mesenteric vein. There is different potentiation exerted by noradrenaline and vasopressin upon the Na+/K(+)-pump activity in veins and in arteries. The data obtained suggest the regional heterogeneity in the Na+/K(+)-pump activity in neurohormone activated blood vessels.

The effect of total saponins from Panax Ginseng C.A. Meyer on the intracellular signalling system in Ehrlich ascites tumor cells.

The mechanisms of action of total saponins from Panax Ginseng C.A. Meyer on the elements of intracellular signalling system in Ehrlich ascites tumor cells were studied.

[The structural differences in the mesenteric vessels of rats with spontaneous (SHR) and DOCA-salt hypertension].

Electron microscopy study of different diameter and functional purpose mesenterial arteries revealed that spontaneous and DOCA-saline hypertension cause different ultrastructural changes in all coats with the exception of cranial mesenterial artery, in endotheliocytes of which no changes occurred in spontaneous hypertension. In cranial mesenterial artery both forms of hypertension cause disorder of normal media structure while in ramifications-only in spontaneous hypertension. Cranial mesenterial artery loses is properties in both forms of hypertension and in ramifications, in contrast, growth of elastic material is observed.

[The effect of chemical sympathectomy on the adrenergic innervation of the blood vessels belonging to different organs in salt-sensitive and salt-resistant Dahl-strain rats].

The action of chemical desympathization by 6-hydroxydophamine influencing adrenergic innervation in different organs vessels (mesenterial, renal and femoral) was studied using histochemical fluorescent method in salt susceptible and salt-resistant Dahl rats kept on a low- and high-salt diet. Heterogeneity of adrenergic innervation of different region vessels was established to display and 6-hydroxydophamine caused complete desympathization only in mesenterial artery and in all veins, while in renal artery retained adrenergic plexus its certain branching and femoral artery-in two thirds of its surface. The process of reinnervation in vessels running with different intensity in different animals and in different vessels was found to start in vessels of majority of animals on day 5-6 after the chemical desympathization.

[Mechanism of activation of Ehrlich ascites carcinoma cells using the total fraction of saponins from Korean ginseng].

Molecular mechanisms of action of the total fraction of saponins from Red Korean Ginseng on the elements of the intracellular signalling system of the cells of ascitic Ehrlich carcinoma (AEC) were studied. The action of the total fraction of the saponins on the AEC cells was compared with that of the classic activator of such cells i.e.

[The activity of the sodium-potassium pump and the ion and water levels in blood vessels with different morphofunctional properties in rats].

Differences were revealed between both arteries and veins as well as among arteries and among veins in the activity of their sodium-potassium pump and ions contents. The data obtained suggest that a heterogeneity specific for the blood vessels is preserved at the level of ion mechanisms, too.

[The effect of chemical sympathectomy with 6-hydroxydopamine on the activity of the sodium-potassium pump and on the contractile responses of the mesenteric artery and vein].

After chemical sympathectomy of mesenteric arteries and veins, different increase of responsiveness to noradrenaline, potassium chloride and ouabain was found. Stronger changes occurred in the vein which seems to be due to different adrenergic innervation of arteries and veins.

[The reactivity of the mesenteric arteries and vein to exposure to endogenous noradrenaline in normal rats and rats with DOCA-salt hypertension].

The DOCA-salt hypertension was shown to induce changes of different types in reactivity of the mesenteric artery and vein, acting on the contractility of the vessels rather than on the sensitivity of their receptor apparatus. The observed heterogeneity of the alpha-adrenoreceptors in the artery and the vein suggests that the artery's reactivity is under the control of both humoral and neurogenic factors, whereas the vein's reactivity is neurogenically controlled rather.

[Homeostatic responses of the body during infusion of UV-irradiated autologous plasma].

Complex examination of changes in some structural and metabolic values in intact dogs after a single infusion of autoplasma irradiated with ultraviolet rays suggests that mobilization of the system of antioxidant protection, inducing a specific reorganisation of the metabolic system, is the trigger mechanism of the organism's adaptation reaction.