Publications by authors named "LE-LE Qiu"
Chinese hamster ovary (CHO) cells represent the most widely utilized host system for industrial production of high-quality recombinant protein therapeutics. Novel CHO cell line development is achieved through genetic and cellular engineering approaches, effectively addressing limitations such as clonal variation and productivity loss during culture. Previous studies have established that MAT2A inhibition in tumor cells promotes expression of the cyclin-dependent kinase inhibitor p21, inducing antitumor activity.
View Article and Find Full Text PDFA novel dual-epigenetic inhibitor enhances recombinant monoclonal antibody expression in CHO cells.
Appl Microbiol Biotechnol
September 2024
Article Synopsis
- Epigenetic regulation is crucial for controlling cellular processes like growth and cell death, and small molecule modulators can fine-tune gene expression in these processes.
- The study found that the dual-HDAC/LSD1 inhibitor I-4 significantly boosted monoclonal antibody production in CHO cells, leading to nearly double the antibody titer despite causing cell cycle arrest.
- Results indicate that I-4 enhances protein expression by increasing histone acetylation and downregulating the HDAC5 gene, demonstrating a new approach to improve recombinant protein yields.
Due to their high-quality characteristics, Chinese hamster ovary (CHO) cells have become the most widely used and reliable host cells for the production of recombinant therapeutic proteins in the biomedical field. Previous studies have shown that the m6A reader YTHDF3, which contains the YTH domain, can affect a variety of biological processes by regulating the translation and stability of target mRNAs. This study investigates the effect of YTHDF3 on transgenic CHO cells.
View Article and Find Full Text PDFArticle Synopsis
- CHO cells can be genetically engineered to boost their ability to produce therapeutic proteins, with recent focus on cell cycle and autophagy regulation to improve yields.
- The study explored the small-molecule compound apilimod, which positively impacts recombinant protein expression by blocking the cell cycle at the G0/G1 phase.
- Apilimod treatment altered the expression of key cell cycle regulators and reduced lysosome biogenesis and autophagy, indicating its potential as an enhancer for protein production in CHO cells.
CHO cells are the preferred host for the production of complex pharmaceutical proteins in the biopharmaceutical industry, and genome engineering of CHO cells would benefit product yield and stability. Here, we demonstrated the efficacy of a Dnmt3a-deficient CHO cell line created by CRISPR/Cas9 genome editing technology through gene disruptions in Dnmt3a, which encode the proteins involved in DNA methyltransferases. The transgenes, which were driven by the 2 commonly used CMV and EF1α promoters, were evaluated for their expression level and stability.
View Article and Find Full Text PDFArticle Synopsis
- - Dunaliella salina, a salt-tolerant marine alga, serves as a crucial model for understanding how extremophiles survive and their practical uses.
- - The study utilized 2D-DIGE and MALDI-TOF/TOF MS to analyze protein expression under different salt concentrations, identifying 141 protein spots with significant changes.
- - Key proteins linked to stress responses, photosynthesis, respiration, and amino acid synthesis were identified, indicating their roles in managing salt stress and maintaining cellular functions.
The aim of this study was to investigate the protective effects of Xin Mai Jia (XMJ) on atherosclerosis (AS) in rabbits and to explore the underlying mechanisms in order to provide experimental evidence for the clinical application of XMJ. An intraperitoneal injection of vitamin D3, combined with a high-fat diet and sacculus injury, was utilized to establish the AS rabbit model. Following the oral administration of lovastatin, Zhibituo and different dosages of XMJ, respectively, blood was drawn from each rabbit for the detection of blood rheological indicators, such as serum lipids.
View Article and Find Full Text PDF