Mechanism of microbial action of the inoculated nitrogen-fixing bacterium for growth promotion and yield enhancement in rice (Oryza sativa L.).

The use of nitrogen-fixing bacteria in agriculture is increasingly recognized as a sustainable method to boost crop yields, reduce chemical fertilizer use, and improve soil health. However, the microbial mechanisms by which inoculation with nitrogen-fixing bacteria enhance rice production remain unclear. In this study, rice seedlings were inoculated with the nitrogen-fixing bacterium R3 (Herbaspirillum) at the rhizosphere during the seedling stage in a pot experiment using paddy soil.

Executive function, limbic circuit dynamics and repetitive and restricted behaviors in children with autism spectrum disorder.

Objective: Repetitive and restricted behaviors (RRBs) are a core symptom of autism spectrum disorder (ASD), but effective treatment approaches are still lacking. Executive function (EF) has been identified as a promising target, as research increasingly shows a link between EF deficits and the occurrence of RRBs. However, the neural mechanisms that connect the two remain unclear.

A dual action electrochemical molecular imprinting sensor based on FeCu-MOF and RGO/PDA@MXene hybrid synergies for trace detection of ribavirin.

In this study, we designed a molecularly imprinted electrochemical sensor based on the reduced graphene oxide/polydopamine@Mxene (RPM) and FeCu-MOF for the detection of antiviral drug ribavirin (RBV). The RPM composite enhances the active surface area and electron transport capacity of the sensor, and the incorporation of FeCu-MOF can not only further improve the catalytic performance of the material, but also enables the sensor to harness the electrical reduction signal of HO. Furthermore, we developed an optimized molecularly imprinted polymer via density functional theory (DFT) to enhance the sensor's specificity and sensitivity for RBV detection.

Simultaneous Unbinned Differential Cross-Section Measurement of Twenty-Four Z+jets Kinematic Observables with the ATLAS Detector.

Z boson events at the Large Hadron Collider can be selected with high purity and are sensitive to a diverse range of QCD phenomena. As a result, these events are often used to probe the nature of the strong force, improve Monte Carlo event generators, and search for deviations from standard model predictions. All previous measurements of Z boson production characterize the event properties using a small number of observables and present the results as differential cross sections in predetermined bins.

Phosphate Limitation Enhances Heterologous Enzyme Production in : Mechanistic Insights and Universal Applicability.

is one of the commonly used hosts for heterologous enzyme expression, depending on media rich in carbon, nitrogen, and phosphate sources for optimal growth and enzyme production. Interestingly, our investigation of maltotetraose-forming amylase, a key enzyme for efficient maltotetraose synthesis, revealed that phosphate limitation significantly enhances the growth rate and production of heterologous enzymes in recombinant . Under phosphate-limited conditions in a 15 L fermenter, the enzyme activity reached 679.

Trends in the incidence and mutational landscape of advanced uterine cancer.

Objective: The aim of this study was to examine disparities in 20-year incidence trends and mutations in advanced-stage uterine cancer in the United States, given poor survival rates.

Methods: Data were obtained from the United States Cancer Statistics for patients from 2001 to 2019 with International Federation of Gynecology and Obstetrics 2009 stage IVA and IVB uterine cancer. SEER∗Stat 8.

Sophoricoside Inhibited Glioblastoma Cell Progression Through Activated AMP-Activated Protein Kinase (AMPK).

Glioblastoma (GBM) is the most common malignant primary brain tumor, with a mean survival of less than 2 years. Unique brain structures and the microenvironment, including blood-brain barriers, put great challenges on clinical drug development. Sophoricoside (Sop), an isoflavone glycoside isolated from seeds of Sophora japonica L.

Long-chain acyl-CoA synthetase regulates systemic lipid homeostasis via glycosylation-dependent lipoprotein production.

Interorgan lipid transport is crucial for organism development and the maintenance of physiological function. Here, we demonstrate that long-chain acyl-CoA synthetase (dAcsl), which catalyzes the conversion of fatty acids into acyl-coenzyme As (acyl-CoAs), plays a critical role in regulating systemic lipid homeostasis. dAcsl deficiency in the fat body led to the ectopic accumulation of neutral lipids in the gut, along with significantly reduced lipoprotein contents in both the fat body and hemolymph.

Fabrication of Dual-Ligand Zn-MOFs with Asynchronous Fluorescence Response for Efficient Ratiometric/Visual Sensing of Tetracycline Antibiotics in Animal-Derived Foods.

The extensive use of tetracyclines in livestock poses health risks due to their residues in animal-derived food; therefore, developing simple detection methods to replace complex traditional approaches is of paramount importance. Here, we developed a dual-ligand zinc-based metal-organic framework material, Zn-BTC-BDC-NH (denoted as ZTD), for the detection of tetracyclines. The intrinsic blue fluorescence of ZTD was quenched upon the introduction of tetracyclines due to electron transfer from -NH of ZTD to -CO- and -OH groups of tetracycline molecules; meanwhile, the new green fluorescence emission was generated through π-π stacking between aromatic rings and the formation of complexes between Zn and C-O/C═O groups.

Preparation and performance characterization of antimicrobial films based on chitosan-nisin-nanocrystalline cellulose and its preservation effect applied to baby cabbage.

Environmental concerns stemming from the widespread use of polyethylene packaging and the perishability of fresh products have promoted the development of antimicrobial biodegradable packaging films in preservation of vegetables. In this study, antimicrobial films based on chitosan (CS)-nisin (Ni)-nanocrystalline cellulose (NCC) were characterized, and its preservation effect applied to baby cabbage was investigated. The results suggest that 1 % CS-0.

Associations of the serum 25-hydroxyvitamin D with mortality among patients in osteopenia or osteoporosis.

Purpose: The correlation between serum vitamin D and mortality in patients with osteopenia or osteoporosis remains unclear. Therefore, this study examined the relationship between serum 25-hydroxy vitamin D [(25(OH)D] and mortality in patients with osteopenia or osteoporosis.

Methods And Result: This prospective cohort study included patients with osteopenia or osteoporosis from the National Health and Nutrition Examination Survey from 2001 to 2018.

Boron-dependent autoinducer-2-mediated quorum sensing stimulates the Cr(VI) reduction of Leucobacter chromiireducens CD49.

Traditionally, abiotic factors such as pH, temperature, and initial Cr(VI) concentration have been undoubtedly recognized as the external driving forces that dramatically affect the microbial-mediated remediation of Cr(VI) pollutants. However, concentrating on whether and how the biological behaviors and metabolic activities drive the microbial-mediated Cr(VI) detoxification is a study-worthy but little-known issue. In this study, Leucobacter chromiireducens CD49 isolated from heavy-metal-contaminated soil was identified to tolerate 8000.

Online monitoring of water quality in industrial wastewater treatment process based on near-infrared spectroscopy.

Water quality monitoring is one of the critical aspects of industrial wastewater treatment, which is important for checking the treatment effect, optimizing the treatment technology and ensuring that the water quality meets the standard. Chemical oxygen demand (COD) is a key indicator for monitoring water quality, which reflects the degree of organic matter pollution in water bodies. However, the current methods for determining COD values have drawbacks such as slow speed and complicated operation, which hardly meet the demand of online monitoring.

Screening of Solvent Systems for Countercurrent Chromatography Separation of Polar Constituents from L. Seeds.

The separation of large polar constituents presents a substantial challenge in natural product research when employing column chromatography techniques, as the process is both complex and time-consuming. In this study, an acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturated sodium chloride solvent system was developed and utilized for the countercurrent chromatography of polar constituents from L. seeds.

Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber Wilt.

strain PJH16, isolated and tested by our team, suppresses cucumber wilt as an efficient biocontrol agent. For further investigation, the strain has been combined with two other ( VJH504 and JNF2) to enhance biocontrol ability, which formed high-efficiency microbial agents in the current study. The methodological target taken is based on achieving the optimal growth conditions of the combined microbial agents; hence, the medium composition and culture conditions were optimized through a single-factor test, orthogonal test and response surface methodology.

Feasibility of Ex Vivo Ligandomics.

We developed ligandomics for the in vivo profiling of vascular ligands in mice, discovering secretogranin III (Scg3) as a novel angiogenic factor that selectively binds to retinal vessels of diabetic but not healthy mice. This discovery led to the development of anti-Scg3 therapy for ocular vasculopathies. However, in vivo ligandomics requires intracardial perfusion to remove unbound phage clones, limiting its use to vascular endothelial cells (ECs).

Isolation and Evaluation of Strains from Traditional Rice Wine Starters (): Enzyme Activities, Antioxidant Capacity, and Flavour Compounds.

Seventy-eight autochthonous strains of were isolated from rice wine starter samples across twenty-nine regions in China to evaluate their potential in traditional rice wine fermentation. Strains were assessed for enzyme activity, antioxidant properties, amino acid production, and volatile flavour compounds. Significant variation in enzyme activities was observed, with acidic protease activity ranging from 280 to 1023 U/g, amylase from 557 to 1681 U/g, and esterase from 370 to 2949 U/g.

Curvularin derivatives from hydrothermal vent sediment fungus Penicillium sp. HL-50 guided by molecular networking and their anti-inflammatory activity.

Guided by molecular networking, nine novel curvularin derivatives (1-9) and 16 known analogs (10-25) were isolated from the hydrothermal vent sediment fungus Penicillium sp. HL-50. Notably, compounds 5-7 represented a hybrid of curvularin and purine.

The Effects of Disinfection Byproduct 2,6-Dichloro-1,4-benzoquinone on the Cyanobacterium : From the Perspectives of Biochemistry and Non-Targeted Metabolomics.

2,6-Dichloro-1,4-benzoquinone (2,6-DCBQ) is an emerging chlorinated disinfection byproduct (DBP) in bodies of water. However, this compound poses an unknown toxic effect on cyanobacteria. In this study, the toxicological mechanisms of 2,6-DCBQ in () were investigated through physiological and nontargeted metabolomic assessments.

The role of disulfide bonds in L-arginine ameliorating the quality of low-salt sturgeon surimi gels induced by microwave: Increasing the diameter and fractal dimension of network.

The purpose of this study was to investigate the mechanism of enhancing gelling properties of low-salt surimi by utilizing the complementary advantages of L-arginine (L-Arg) and microwave (MW) from the perspective of gels' network characteristics. At MW 3 min, the diameters of protein filaments were increased from 0.015 μm to 0.

Photoacoustic Imaging with Attention-Guided Deep Learning for Predicting Axillary Lymph Node Status in Breast Cancer.

Rationale And Objectives: Preoperative assessment of axillary lymph node (ALN) status is essential for breast cancer management. This study explores the use of photoacoustic (PA) imaging combined with attention-guided deep learning (DL) for precise prediction of ALN status.

Materials And Methods: This retrospective study included patients with histologically confirmed early-stage breast cancer from 2022 to 2024, randomly divided (8:2) into training and test cohorts.

Association of Circulating Phenylacetylglutamine With Multi-Vessel Coronary Disease Severity and Outcomes in ST-Segment-Elevation Myocardial Infarction.

Background: There is a lack of evidence regarding the association between plasma phenylacetylglutamine levels and lesion severity and clinical prognosis in patients with ST-segment elevation myocardial infarction (STEMI) with multivessel coronary disease (MVCD). This study aims to investigate the potential of phenylacetylglutamine as a biomarker for major adverse cardiovascular events (MACEs) of patients with STEMI and MVCD.

Methods And Results: Clinical data and blood samples were collected from 631 patients with STEMI and MVCD, who underwent primary percutaneous coronary intervention.

A Phosphate-Starvation Enhanced Purple Acid Phosphatase, GmPAP23 Mediates Intracellular Phosphorus Recycling and Yield in Soybean.

Plant internal phosphorus (P) recycling is a complex process, which is vital for improving plant P use efficiency. However, the mechanisms underlying phosphate (Pi) release from internal organic-P form remains to be deciphered in crops. Here, we functionally characterised a Pi-starvation responsive purple acid phosphatase (PAP), GmPAP23 in soybean (Glycine max).

Retention and migration of microplastics in stepped paddy fields: A study on microplastic dynamics in the special irrigation system.

The stepped paddy fields (SPFs) are important for food security and sustainable development. The unique spatial structure and complex hydrological processes in this system make it difficult to understand the migration of pollutants. In this study, microplastic pollution was investigated in the water and soil from Ziquejie SPFs, China.