Enhancing High-Level Food-Grade Expression of Glutamate Decarboxylase and Its Application in the Production of γ-Aminobutyric Acid.

Gamma-aminobutyric acid (GABA), a non-proteinogenic amino acid, exhibits diverse physiological functions and finds extensive applications in food, medicine, and various industries. Glutamate decarboxylase (GAD) can effectively convert L-glutamic acid (L-Glu) or monosodium glutamate (MSG) into GABA. However, the low food-grade expression of GAD has hindered large-scale GABA production.

A legume-enriched diet improves metabolic health in prediabetes mediated through gut microbiome: a randomized controlled trial.

Healthy dietary patterns rich in legumes can improve metabolic health, although their additional benefits in conjunction with calorie restriction have not been well-established. We investigated effects of a calorie-restricted, legume-enriched, multicomponent intervention diet compared with a calorie-restricted control diet in 127 Chinese prediabetes participants, living in Singapore. The study was a 16-week, single-blind, parallel-design, randomized controlled trial (n = 63 intervention group (IG), n = 64 control group (CG); mean ± SD age 62.

Magnetic field-mediated oxidative modification of myoglobin: One effective method for improving the gel properties of myofibrillar protein.

This study employed a magnetic field to investigate the impact of myoglobin (Mb) oxidation (0-20 mmol/L HO) on the gel properties of myofibrillar protein (MP). The results indicated that magnetic field could further facilitate the rearrangement of the Mb structure, resulting in the transfer of its internal reactive groups to the external environment. This contributed to hydration and cross-linking between MP.

A Glutamate Receptor-Like Gene AtGLR25 With Its Unusual Splice Variant Has a Role in Mediating Glutamate-Elicited Changes in Arabidopsis Root Architecture.

The occurrence of external L-glutamate at the Arabidopsis root tip triggers major changes in root architecture, but the mechanism of -L-Glu sensing is unknown. Members of the family of GLUTAMATE RECEPTOR-LIKE (GLR) proteins are known to act as amino acid-gated Ca-permeable channels and to have signalling roles in diverse plant processes. To investigate the possible role of GLRs in the root architectural response to L-Glu, we screened a collection of mutants with T-DNA insertions in each of the 20 AtGLR genes.

N-glycosylation-modifications-driven conformational dynamics attenuate substrate inhibition of d-lactonohydrolase.

Achieving enzyme catalysis at high substrate concentrations is a substantial challenge in industrial biocatalysis, and the role of glycosylation in post-translational modifications that modulate enzyme substrate inhibition remains poorly understood. This study provides insights into the role of N-glycosylation in substrate inhibition by comparing the catalytic properties of d-lactonohydrolase (d-Lac) derived from Fusarium moniliforme expressed in prokaryotic and eukaryotic hosts. Experimental evidence indicates that recombinant d-Lac expressed in Pichia pastoris (PpLac-WT) exhibits higher hydrolysis rates at a substrate concentration of 400 g/L, with reduced substrate inhibition and enhanced stability compared to the recombinant d-Lac expressed in Escherichia coli (EcLac-WT).

Multi-omics reveals mechanism of hydroxylamine-enhanced ultimate nitrogen removal in pilot-scale anaerobic/aerobic/anoxic system.

Hydroxylamine (HA) dosing is an effective strategy for promoting partial nitrification (PN); however, its impact on endogenous denitrification remains underexplored. In this study, long-term continuous HA dosing (1.4 mg/L) was introduced for over 110 days in a pilot-scale anaerobic/aerobic/anoxic (AOA) system treating municipal wastewater (66.

Tenecteplase Thrombolysis for Stroke up to 24 Hours After Onset With Perfusion Imaging Selection: The CHABLIS-T II Randomized Clinical Trial.

Background: Whether it is effective and safe to extend the time window of intravenous thrombolysis up to 24 hours after the last known well is unknown. We aimed to determine the efficacy and safety of tenecteplase in Chinese patients with acute ischemic stroke due to large/medium vessel occlusion within an extended time window.

Methods: Patients with ischemic stroke presenting 4.

Population Pharmacokinetics and Dosing Optimization of Norvancomycin for Chinese Patients with Community-Acquired Pneumonia.

Purpose: Determining the optimal dosage of norvancomycin (NVCM) for Chinese patients with community-acquired pneumonia (CAP) caused by gram-positive cocci remains uncertain. This research aimed to identify influential factors affecting NVCM pharmacokinetics and explore optimal dosage regimens via population pharmacokinetic (PPK) analysis.

Patients And Methods: A prospective analysis was conducted at the Second Hospital of Hebei Medical University (Shijiazhuang, China).

Advances in biopharmaceutical products for hemophilia.

Hemophilia is caused by the deficiency of clotting factors due to a single genetic abnormality. Replacement therapies have evolved from plasma-derived to recombinant coagulation factor concentrates but continue to have certain limitations. Monoclonal antibodies are clinical prophylactic treatment options unaffected by inhibitors and have better compliance than coagulation factor concentrates for patients with hemophilia.

[Spatial Relationship between Eco-environment Quality and Land Use Change in Guangdong Province-Hong Kong-Macao, China Based on Remote Sensing-based Ecological Index].

The relationship between eco-environment quality and land use change is an important guarantee for regional sustainable development. Guangdong Province-Hong Kong-Macao have always been closely linked and are now entering a period of accelerated development, but there is a lack of research on the spatial relationship between eco-environment quality and land use change integrated among the three places. Based on historical land use data and Google earth engine （GEE）, MODIS images from the summer of 2000 to 2020 （from June 1 to September 1） were selected （with a time span of five years）.

Analysis on Degradation Mechanism and Product Recycle of Ex-service Wind Turbine Blades by Hydrothermal Liquefaction.

The recycling of ex-service wind turbine blades (EWTBs) presents a significant challenge for the future. Hydrothermal liquefaction (HTL) has emerged as a promising approach for the recovery of resins and glass fibers (GFs) from EWTBs. This study offers a comprehensive analysis of the separation mechanisms and product characteristics under the catalytic effect of an acidic medium during the HTL tests.

Combined carbapenem resulted in a 4.48-fold increase in valproic acid clearance: a population pharmacokinetic model in Chinese children and adults with epilepsy or after neurosurgery.

Our study aims to explore the pharmacokinetics of valproic acid (VPA) in Chinese patients with epilepsy or after neurosurgery and establish a robust population pharmacokinetics (PPK) model. The PPK model was developed using nonlinear mixed-effects modeling, incorporating a total of 615 VPA plasma concentration data points from 443 Chinese epilepsy or after neurosurgery patients. A one-compartment model with an additive residual model was established.

Population pharmacokinetics of long-term hydroxychloroquine therapy in patients with rheumatic diseases.

Aims: Hydroxychloroquine (HCQ) is recommended for the long-term treatment of rheumatic diseases such as rheumatoid arthritis and systemic lupus erythematosus. Given the complex process of HCQ metabolism and individual physiological differences, the metabolic profile of HCQ after long-term administration is unknown. This study aimed to establish a population pharmacokinetic model for long-term HCQ treatment in patients with rheumatic diseases and to identify the factors influencing HCQ metabolism.

Population pharmacokinetics and dosing simulations of meropenem in septic critically ill patients with complicated intra-abdominal infection or pneumonia.

Objectives: Meropenem pharmacokinetics (PK) may be altered in septic critically ill patients with complicated intra-abdominal infections (cIAI) and pneumonia. We aimed to evaluate the covariates affecting meropenem PK and the performance of different dosing regimens to optimize the PK/pharmacodynamic target.

Methods: Population PK analysis was performed using non-linear mixed-effects modeling.

Pharmacokinetics and safety of colistin sulfate after single and multiple intravenous doses in healthy Chinese subjects.

Objective: Increasing antimicrobial resistance has led to the revival of the polymyxins as a last-resort therapeutic option for multidrug-resistant Gram-negative bacterial infections. A parenteral formulation of colistin sulfate is available solely in China. While the onset of action of IV colistin may occur faster than with its prodrug CMS, its pharmacokinetic (PK) profile remains unclear.

Induced electric field as alternative pasteurization to improve microbiological safety and quality of bayberry juice.

Recently, unconventional techniques like induced electric field (IEF) for continuous pasteurization of liquid food have received great attention. In this study, the effect of IEF on temperature rise, microbiological and quality characteristics of bayberry juice was investigated. Voltage, current, and flow rate affected the terminal temperature.