Semi-Targeted Profiling of the Lipidome Changes Induced by in Disease-Resistant and L. Varieties.

The ascomycete is a serious pathogen in viticulture. Despite the fact that some grapevine genotypes exhibit mono-locus or pyramided resistance to this fungus, the lipidomics basis of these genotypes' defense mechanisms remains unknown. Lipid molecules have critical functions in plant defenses, acting as structural barriers in the cell wall that limit pathogen access or as signaling molecules after stress responses that may regulate innate plant immunity.

Secondary and primary metabolites reveal putative resistance-associated biomarkers against in resistant grapevine genotypes.

Numerous fungicide applications are required to control , the causative agent of powdery mildew. This increased demand for cultivars with strong and long-lasting field resistance to diseases and pests. In comparison to the susceptible cultivar 'Teroldego', the current study provides information on some promising disease-resistant varieties (mono-locus) carrying one resistant locus: BC4 and 'Kishmish vatkana', as well as resistant genotypes carrying several resistant loci (pyramided): 'Bianca', F26P92, F13P71, and NY42.

Mono-Locus and Pyramided Resistant Grapevine Cultivars Reveal Early Putative Biomarkers Upon Artificial Inoculation With .

One of the most economically important grapevine diseases is Downy mildew (DM) caused by the oomycete . A strategy to reduce the use of fungicides to compensate for the high susceptibility of is the selection of grapevine varieties showing pathogen-specific resistance. We applied a metabolomics approach to evaluate the metabolic modulation in mono-locus resistant genotypes carrying one locus associated with resistance () (BC4- , Bianca- , F12P160- , Solaris- ), as well as in pyramided resistant genotypes carrying more than one (F12P60- and F12P127- ) taking as a reference the susceptible genotype Pinot Noir.

Two-omics data revealed commonalities and differences between Rpv12- and Rpv3-mediated resistance in grapevine.

Plasmopara viticola is the causal agent of grapevine downy mildew (DM). DM resistant varieties deploy effector-triggered immunity (ETI) to inhibit pathogen growth, which is activated by major resistance loci, the most common of which are Rpv3 and Rpv12. We previously showed that a quick metabolome response lies behind the ETI conferred by Rpv3 TIR-NB-LRR genes.

Emergent Ascomycetes in Viticulture: An Interdisciplinary Overview.

The reduction of pesticide usage is a current imperative and the implementation of sustainable viticulture is an urgent necessity. A potential solution, which is being increasingly adopted, is offered by the use of grapevine cultivars resistant to its main pathogenic threats. This, however, has contributed to changes in defense strategies resulting in the occurrence of secondary diseases, which were previously controlled.