The structure of rusticyanin, a protein with a molecular weight of 16.8 kDa, has recently been determined using direct methods and single-wavelength anomalous scattering (SAS).
A comparison was made between results obtained from the Sim distribution approach and the CCP4 procedure MLPHARE against direct methods.
The analysis revealed that direct methods provided significantly better phase accuracy and improved electron-density maps, with an average phase error of 56 degrees compared to around 63 degrees for the other approaches.