Publications by authors named "L Yu Novikova"
Introduction: Alzheimer's disease (AD) features changes in mitochondrial structure and function. Investigators debate where to position mitochondrial pathology within the chronology and context of other AD features.
Methods: To address whether mitochondrial dysfunction alters AD-implicated genes and proteins, we treated SH-SY5Y cells and induced pluripotent stem cell (iPSC)-derived neurons with chloramphenicol, an antibiotic that inhibits mtDNA-generated transcript translation.
PI primers increase the efficacy of LAMP and RT-LAMP for SARS-CoV-2 and MS2 phage detection.
Diagn Microbiol Infect Dis
November 2024
LAMP (Loop-mediated isothermal amplification) is a popular method for the molecular diagnostics of numerous pathogens, specifically useful for point-of-care testing. However, the efficacy and sensitivity of LAMP still need to be maximised for the best performance in clinical settings. Adding a novel fourth primer pair is a promising way to accelerate the LAMP speed.
View Article and Find Full Text PDFHuman adenoviruses (HAdVs) are common pathogens that are associated with a variety of diseases, including respiratory tract infections (RTIs). Without reliable, fast, and cost-effective detection methods for HAdVs, patients may be misdiagnosed and inappropriately treated. To address this problem, we have developed a multiplex loop-mediated isothermal amplification (LAMP) assay for the detection of the species (HAdV-B), (HAdV-C) and (HAdV-E) that cause RTIs.
View Article and Find Full Text PDFArticle Synopsis
- Pregnenolone and progesterone are crucial steroid hormones in mammals, and new recombinant strains are needed for more efficient microbiological production from sterols.
- A new strain of Mycolicibacterium smegmatis was developed to express steroidogenic enzymes from the bovine adrenal cortex, leading to the production of progesterone and pregnenolone from cholesterol.
- The study demonstrated successful transformation of sterols under optimized conditions, yielding high-purity crystalline pregnenolone, which enhances the potential for efficient hormone production using microbial platforms.
Background: Some epidemiologic studies associate traumatic brain injury (TBI) with Alzheimer's disease (AD).
Objective: To test whether a TBI-induced acceleration of age-related mitochondrial change could potentially mediate the reported TBI-AD association.
Methods: We administered unilateral controlled cortical impact (CCI) or sham injuries to 5-month-old C57BL/6J and tau transgenic rTg4510 mice.