Developing a highly efficient 4-hydroxyphenylacetate-3-hydroxylase for salvianic acid A synthesis by computer-aided molecular modification.

Salvianic acid A (SAA) is a catechol compound known for its diverse physiochemical functions and has significant applications in the food and pharmaceutical industries. 4-Hydroxyphenylacetate-3-hydroxylase (4HPA3H) is a critical enzyme for SAA biosynthesis, and improving its activity towards p-hydroxyphenyllactate acid (4HPLA) is essential for highly efficient SAA production in stable biosynthetic pathways. To address this, the distal site and loops of the substrate pocket were modified to improve 4HPA3H catalytic activity towards 4HPLA using computer-aided molecular modification methods.

Structural characteristics of linear dextrin and in vitro digestion of lauric acid complexes generated via gradient alcohol precipitation.

Linear dextrin (LD), a low-molecular-weight carbohydrate, regarded as a type of short amylose, can form resistant starch when combined with fatty acids. This study investigated the structural properties and in vitro digestion of linear dextrins and their complexes with lauric acid (LA). Four groups of linear dextrins were prepared by pretreating gelatinized high-amylose maize starch (HAMS) with pullulanase followed by gradient ethanol precipitation at concentrations of 0 %, 50 %, 60 %, and 70 % (v/v).

Combined anterior and posterior miniscrews increase apical root resorption of maxillary incisors in protrusion and premolar extraction cases.

Objective: Miniscrews are commonly utilized as temporary anchorage devices (TADs) in cases of maxillary protrusion and premolar extraction. This study aimed to investigate the effects and potential side effects of two conventional miniscrew configurations on the maxillary incisors.

Methods: Eighty-two adult patients with maxillary dentoalveolar protrusion who had undergone bilateral first premolar extraction were retrospectively divided into three groups: non-TAD, two posterior miniscrews only (P-TADs), and two anterior and two posterior miniscrews combined (AP-TADs).

Maternal and neonatal outcomes in pregnant women undergoing cardiac surgery: a systematic review and meta-analysis.

Objective: Cardiac diseases that require surgical intervention present a unique challenge during pregnancy and may affect both maternal and neonatal outcomes. This systematic review and meta-analysis aimed to evaluate maternal and neonatal outcomes in pregnant females undergoing cardiac surgery.

Methods: A comprehensive manual and electronic search was conducted in PubMed, EMBASE, Cochrane Library, and Web of Sciences databases for studies published up to 31 May 2024.

Cost-effectiveness analysis of pembrolizumab plus chemotherapy versus chemotherapy in untreated advanced pleural mesothelioma in the Chinese healthcare system.

Objective: The combination of pembrolizumab and chemotherapy has demonstrated notable clinical advantages in improving overall survival than chemotherapy alone for patients with untreated advanced pleural mesothelioma. The purpose of this study was to assess its cost-effectiveness.

Materials And Methods: A Markov state-transition model was constructed using data from the IND227 phase 3 randomized clinical trial.