Publications by authors named "L W Chambers"
Background: The etiology of nongonococcal urethritis (NGU) is incompletely understood. We sought to determine if genitourinary bacterial diversity or specific taxa were associated with incident NGU.
Methods: From August 2014-July 2018, men who have sex with women attending a sexual health clinic were clinically evaluated, including Mycoplasma genitalium (MG) and Chlamydia trachomatis (CT) testing, at enrollment and six monthly visits.
Article Synopsis
- Emergency department visits present a key opportunity for delivering prevention services to individuals at high risk of opioid overdose, particularly by assessing their "recovery capital."
- This study analyzed data from 543 patients enrolled in a randomized controlled trial in Rhode Island, focusing on the relationship between recovery capital (measured by the BARC-10) and subsequent treatment engagement and overdose risk.
- The results indicated that the majority of patients had low recovery capital (BARC-10 score <47), and there was no significant association found between recovery capital and either 30-day treatment engagement or the risk of non-fatal or fatal overdoses within 18 months.
Article Synopsis
- Bacteria have operons similar to eukaryotic ubiquitination pathways, which include proteins involved in adding and removing ubiquitin-like molecules.
- Recent studies indicate some of these pathways help bacteria defend against bacteriophages, although many functions remain unclear.
- This research reveals unique structural diversity in bacterial ubiquitin-like proteins and suggests they form filaments that help bacteria respond to stress through calcium ion interactions.
Ubiquitination is a fundamental and highly conserved protein post-translational modification pathway, in which ubiquitin or a ubiquitin-like protein (Ubl) is typically conjugated to a lysine side chain of a target protein. Ubiquitination is a multistep process initiated by adenylation of the Ubl C-terminus, followed by sequential formation of 2-3 Ubl~cysteine thioester intermediates with E1, E2, and E3 proteins before formation of the final Ubl-lysine isopeptide bond. Ubiquitination is conserved across eukaryotes, and recent work has also revealed at least two related bacterial pathways that perform protein conjugation in the context of antiphage immunity.
View Article and Find Full Text PDFIntroduction: Buprenorphine is a highly effective treatment for opioid use disorder (OUD). However, provider observations and preliminary research suggest that the current standard maintenance dose may be insufficient for suppressing withdrawal and preventing cravings among people who use or have used fentanyl. Buprenorphine dosing guidelines were based on studies among people who use heroin and have not been formally re-evaluated since fentanyl became predominant in the unregulated drug supply.
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