LoRaWAN for Vehicular Networking: Field Tests for Vehicle-to-Roadside Communication.

Vehicular wireless networks are one of the most valuable tools for monitoring platforms in the automotive domain. At the same time, Internet of Things (IoT) solutions are playing a crucial role in the same framework, allowing users to connect to vehicles in order to gather data related to their working cycle. Such tasks can be accomplished by resorting to either cellular or non-cellular wireless technologies.

Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors.

In this work, we present the development and biofunctionalization of a fiber-optic ball-resonator biosensor for the real-time detection of vaccinia poxvirus. We fabricated several ball-tip resonators, functionalized through a silanization process to immobilize two bioreceptors: the monoclonal anti-L1R antibody targeting the L1R protein, and the polyclonal rabbit serum antibodies targeting the whole vaccinia virus (VV) pathogen. Experimental measurements were carried out to detect VV in concentrations from 10 to 10 plaque-forming units (PFU), with a limit of detection of around 1.

Mobility Classification of LoRaWAN Nodes Using Machine Learning at Network Level.

LoRaWAN networks rely heavily on the adaptive data rate algorithm to achieve good link reliability and to support the required density of end devices. However, to be effective the adaptive data rate algorithm needs to be tuned according to the level of mobility of each end device. For that purpose, different adaptive data rate algorithms have been developed for the different levels of mobility of end devices, e.

Ultralow Limit Detection of Soluble HER2 Biomarker in Serum with a Fiber-Optic Ball-Tip Resonator Assisted by a Tilted FBG.

An optical-fiber biosensor has been developed for the detection of the breast cancer biomarker soluble human epidermal growth factor receptor-2 (sHER2). The sensor was fabricated by combining a tilted fiber Bragg grating (TFBG) with a ball resonator, allowing us to achieve an excellent sensitivity compared to other optical-fiber-based sensors. The sensor exhibits a resonance comb excited by the TFBG and the spectral profile of the ball resonator.

Rational Engineering of a Sub-Picomolar HIV-1 Blocker.

With the aim of rationally devising a refined and potent HIV-1 blocker, the cDNA of CCL5 5p12 5m, an extremely potent CCR5 antagonist, was fused to that of C37, a gp41-targeted fusion inhibitor. The resulting CCL5 5p12 5m-C37 fusion protein was expressed in and proved to be capable of inhibiting R5 HIV-1 strains with low to sub-picomolar IC, maintaining its antagonism toward CCR5. In addition, CCL5 5p12 5m-C37 inhibits R5/X4 and X4 HIV-1 strains in the picomolar concentration range.