Spectrophotometric determination of 0-50 ng of chromium in 1 ml of human serum.

A method is described for the determination of chromium in 1 ml of human serum or plasma. It is based on a wet decomposition and a spectrophotometric determination with diphenylcarbazide after extraction with methyl isobutyl ketone. A 40-mm cuvette with less than 1 ml sample volume is described.