Publications by authors named "L Valasek"
Article Synopsis
- Mitochondrial shape is crucial for cell health, but assessing it in yeast cells is challenging due to limited methods.
- A new deep learning model called MitoSegNet was developed, outperforming traditional methods in accurately segmenting mitochondria in yeast cells.
- The study revealed unique mitochondrial changes in a specific yeast strain (mmi1Δ) under oxidative stress, and all tools and procedures used are publicly accessible online for further research.
Activating transcription factor 4 (ATF4) is a key player in the integrated stress response, whose expression is subject to tight translational control. Studying its stress-provoked induction, accompanied by the general translational shutdown, is intricate because the expression of reference genes declines rapidly, and finding appropriate normalization controls is challenging. We present a protocol for human hemagglutinin-tagged ATF4 (hATF4-HA) detection and high-throughput quantification in non-stress versus stress conditions using automated and quantitative western blotting.
View Article and Find Full Text PDFProtein synthesis plays a major role in homeostasis and when dysregulated leads to various pathologies including cancer. To this end, imbalanced expression of eukaryotic translation initiation factors (eIFs) is not only a consequence but also a driver of neoplastic growth. eIF3 is the largest, multi-subunit translation initiation complex with a modular assembly, where aberrant expression of one subunit generates only partially functional subcomplexes.
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- Human tRNA modifications at positions 16 and 17, known as D16/D17, are produced by the enzyme DUS1L, which was identified as essential for these modifications in glioblastoma cells.
- Knocking out DUS1L leads to a loss of D16/D17 modifications and negatively impacts cell growth while its overexpression disrupts tRNA processing and translation.
- Higher levels of DUS1L in glioma patients correlate with worse prognoses, highlighting its potential role in cancer biology and the need for further research into its functions.
Recycling of 40S ribosomal subunits following translation termination, entailing release of deacylated tRNA and dissociation of the empty 40S from mRNA, involves yeast Tma20/Tma22 heterodimer and Tma64, counterparts of mammalian MCTS1/DENR and eIF2D. MCTS1/DENR enhance reinitiation (REI) at short upstream open reading frames (uORFs) harboring penultimate codons that confer heightened dependence on these factors in bulk 40S recycling. Tma factors, by contrast, inhibited REI at particular uORFs in extracts; however, their roles at regulatory uORFs in vivo were unknown.
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