[Time course of changes in iron metabolic and oxidative-antioxidative system parameters in patients with acute myeloid leukemia].

Aim: To study an association between iron metabolism, free radical oxidation (FRO), and antioxidative system (AOS) in patients with acute myeloid leukemia (AML) during intensive chemotherapy.

Subjects And Methods: AML patients (n = 14) with a median age of 46 years received 7+3 courses (n = 3) containing cytarabine > or = 1 g/m2/introduction (n = 8) and myeloablative conditioning regimen before hematopoietic stem cell transplantation (n = 3). The concentrations of iron, ferritin, transferrin saturation (TFS), and malonic dialdehyde and the activity of superoxide dismutase (SOD), ceruloplasmin (CP), and catalase were investigated in their sera.

[Non-specific changes in the complement system in neoplastic processes].

The study using immunochemical and biological methods was concerned with assaying complement components C1q, C3, C4, and C5, C3 derivatives--C3a and, C3(H2O), C1ing control protein, hemolytic activity (CH50), total proteolytic activity and regulatory immune complexes in sera from patients with cancer of the stomach, breast and ovary, subjects at risk of cancer and healthy donors. Neoplasia was associated with lowered levels of intact C3 and C1ing matched by high concentrations of C3a, C1q and C4. Hemolytic activity (CH50) was increased against the background of relatively higher total proteolytic activity both in healthy subjects and cancer patients.

[Diagnostic and prognostic significance of blood-serum ceruloplasmin, acetylcholinesterase and total proteolytic activity in patients with multiple myeloma].

The study included 82 patients with multiple myeloma (MM) to evaluate the diagnostic and prognostic significance of correlation between of levels of ceruloplasmin (CP), acetylcholinesterase (ACE) and total proteolytic activity (TPA) in blood serum and immunochemical pattern, tumor mass and response to chemotherapy. It was shown that CP, ACE and TPA determination may be used as additional markers to confirm therapeutic benefits, to timely detect relapse and to verify resistance to chemotherapy. It was also demonstrated that resultant decrease in CP and TPA and increase in ACE levels are reliable indicators of changes developing in MM course and remission fulfillment.

[Certain biochemical features of leukocytes in blast crisis of chronic myelogenous leukemia].

The content of nuclear high mobility group (HMG) proteins, activities of ornithine decarboxylase (ODC), adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) and also glycosaminoglycan (GAG) content and composition were studied in leukocytes of patients with chronic myelogenous leukemia in the phase of blast crisis (BC CML). Myeloid and lymphoid cytochemical variants of BC CML differ by biochemical parameters. It is suggested, that the content of HMG-proteins, activities of ODC and PNP, and electrophoretic patterns of GAGs could be used in diagnostics of two main variants of BC CML.

[Experimental study of the biopreparation Lymphotilin as an antiproliferative and antitumor agent].

We found in experiments involving the use of a biopreparation lymphotilin that its administration was followed by a decrease in proliferative activity of cultured tumor cells and a longer survival of mice bearing transplantable leukemia. An intensified intercalation of ethidium bromide in nucleic acids of tumor cells in lymphotilin culture points to the drug activity on nuclear level. Tumor cell inhibition by lymphotilin holds much promise for the practice of hematology.