Stimulation of protein synthesis in dystrophic brain cortex neurons of hypoxia-subjected rats by proliferation-activating protein isolated from nervous tissue of newborn animals.

Hypoxia causes mass diffuse dystrophy of brain cortex neurons, reduces RNA and protein synthesis in neurons and DNA synthesis in the total brain cortex in adult rats. Subsequent transplantation of embryonic nervous tissue (ENT) into the brain of hypoxia-subjected rats normalizes the structure of a considerable part of dystrophic neurons. A protein activator (molecular mass 30,000 D and PI 6.

Normalization of protein synthesis and the structure of brain dystrophic neurons after the action of hypoxia, 10% NaCl and organ-specific RNA.

It was shown previously (Polezhaev and Alexandrova, 1986) that hypoxic hypoxia causes mass (up to 30%) diffuse dystrophy of brain cortex and hippocamp neurons in rats, disturbances in the higher nervous activity, reduction of protein, RNA synthesis in neurons and of DNA synthesis in the whole brain cortex. Transplantation of embryonic nervous tissue (ENT) in one of the hemispheres normalizes all the above abnormalities observed in some neurologic and mental diseases in humans. However, transplantation may entail injuries of parenchyma and brain blood vessels.

Normalization of protein synthesis in dystrophic neurons of cerebral cortex in rats after hypoxia, opening of the blood-brain barrier and treatment with organospecific RNA.

The possibility of normalization of protein synthesis intensity was explored in dystrophic neurons and in the total brain cortex of rats after acute hypoxic hypoxia. We avoided transplantation of embryonic nervous tissue (ENT) into the rat brain, as we did before, as well as operations and brain damages in opening of the blood-brain barrier (BBB) caused by hypoxia and intramuscular injections of organo (brain)-specific RNA. As shown by the autoradiographic and biochemical methods using radioactive isotopes (3H-leucine), hypoxia causes a statistically significant reduction in the intensity of protein synthesis which increases and becomes normalized after injection of brain-specific RNA into femoral muscles of animals.