[Role of the cytosolic factor in the interaction of [3H] alpha-tocopherol with rat liver nuclei].

A protein fraction (Mr = 30-70 kD) specifically binding [3H]alpha-tocopherol was isolated from rat liver cytosol. Using high performance ion exchange chromatography, this fraction was separated into acid and alkaline protein subfractions. Acid proteins make up to 41% of the total protein pool and they bind the label 8 times more intensively than the alkaline ones.

[Isolation and properties of enzyme preparations from the whale pancreas].

A simple and convenient technique was developed for isolation of the proteolytic enzyme complexes from the whale (Balaenoptera) pancreas. The proposed techniques enables the proteolytic complexes to be obtained with the protein yield 2.6 times higher than the classical procedure.