[Immunogenic and protective characteristics of surface antigens 6 and d of Burkholderia pseudomallei].

Aim: Extraction of complex of Burkholderia pseudomallei antigens 6+d (Ag6+d) and study of its immunogenic and protective characteristics.

Materials And Methods: Studied antigens were obtained from acrtone-dried cells of B. pseudomallei 57576.

[Outcomes of pulmonary tuberculosis in patients without bacterial isolation (according to sputum microscopy data)].

The composition, the outcomes of treatment and a follow-up were analyzed in 4 954 first detected adult patients with pulmonary tuberculosis and the baseline negative results of a sputum test for acid-resistant mycobacteria, with the involvement of not more than a lobe. The basic course of therapy yielded clinical and X-ray effects in 83.6% of cases.

[Compulsory hospitalization of non-compliant patients with tuberculosis].

The procedure and results of forced treatment per curiam were analyzed in 584 bacteria-discharging persons registered as Group 1 patients to be examined in the tuberculosis facilities of the Kemerovo Region in 2004 to 2007. If a package of measures for forcing the patient to be treated is ineffective, a phthisiatrician draws up documents for court. By the decision of the court, the patient is conveyed by justice officers to a tuberculosis hospital.

[Comparative assessment of DNA extraction methods for identification of glanders and melioidosis etiological agents by PCR].

Pathogenic Burkholderia are considered as a cause of dangerous infections and potential agents of bioterrorism. Comparative assessment of different methods of extraction and purification of DNA for PCR analysis of pure cultures and samples contaminated by etiological agents of glanders and melioidosis was performed. Samples of soil and food artificially contaminated by pathogenic Burkholderia as well as organs of infected animals were tested.

[Phagocytosis of Burkholderia mallei as a criterion for immunogenicity evaluation of its capsular antigens].

Test-system using index of phagocytosis of noncapsulated mutant loaded by one of the several capsular antigenic complexes was developed and used for screening for both immunogenic and protective capsular antigens of B. mallei. Direct correlation between index of phagocytosis, level of delayed-type hypersensivity, and protective effect of capsular antigens has been shown on the model of experimental melioidosis in susceptible white mice, guinea pigs and white rats.