The genetic constructions based on integrative vector pGV3850 were used to introduce bacterial genes xyl and T-cyt into potato cells. The transformation was carried out using the leaf-disc method with modifications. A special system for obtaining regenerants from explants of potato in vitro plants or calli has been designed that permitted the selection of transgenic shoots.
View Article and Find Full Text PDFIntegrative expression vector pST6 was developed for dicote plant transformation. The vector contains neomycin phosphotransferase gene under control of PNOS promoter which confers kanamycin resistance to transformed plant cells. The vector also includes expression cassette with strong constitutive 35S CaMV promoter for cloning of foreign genes.
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