Macromolecular interactions and geometrical confinement determine the 3D diffusion of ribosome-sized particles in live cells.

The crowded bacterial cytoplasm is composed of biomolecules that span several orders of magnitude in size and electrical charge. This complexity has been proposed as the source of the rich spatial organization and apparent anomalous diffusion of intracellular components, although this has not been tested directly. Here, we use biplane microscopy to track the 3D motion of self-assembled bacterial genetically encoded multimeric nanoparticles (bGEMs) with tunable size (20 to 50 nm) and charge (-3,240 to +2,700 e) in live cells.

Giant KASH proteins and ribosomes synergistically establish cytoplasmic biophysical properties .

Understanding how cells control their biophysical properties during development remains a fundamental challenge. While cytoplasmic macromolecular crowding affects multiple cellular processes in single cells, its regulation in living animals remains poorly understood. Using genetically encoded multimeric nanoparticles for rheology, we discovered that tissues maintain distinct cytoplasmic biophysical properties that differ from those observed across diverse systems, including bacteria, yeast species, and cultured mammalian cells.

The person-based approach to intervention development: A scoping review of methods and applications.

Background: The person-based approach (PBA) has emerged as a prominent methodology guiding the development of digital and hybrid health behaviour change interventions over the last decade, and there is a salient need to understand its utilization.

Objective: This study aims to describe which elements of the PBA have been utilised in intervention development research, for which populations, and how this has been reported.

Methods: A search for intervention development papers published between 2015 and 2023 using forward citation searches was undertaken in Scopus, using two seed articles.

Barriers and facilitators to implementing comprehensive sex education in Texas public schools: A qualitative study.

Introduction: In Texas, the adolescent birth rate is higher than the national average and STIs have steadily increased over the last decade. Sex education is not mandated in Texas and the majority of public schools provide an abstinence-based sex education. Comprehensive sexuality education (CSE) programs are widely endorsed by national and global health organizations and research has shown that they are more effective in reducing poor sexual health outcomes than abstinence-based programs.

E-cigarette Quit Attempts in Emerging Adults: Motivations for Attempts and Predictors of Cessation Barriers.

Introduction: Although young adults use electronic nicotine delivery systems (ENDS) more often than any other demographic group, most are interested in cessation; however, little is known about their cessation experiences. The present study examined characteristics associated with quit attempts, reasons for quitting and resources utilized, and psychological symptoms [i.e.