Post-Chornobyl remote radiation effects on human sperm and seminal plasma characteristics.

Aim: The research was aimed on analysis of the remote consequences of Chornobyl accident on the reproductive function of men adult residing in Ukraine.

Materials And Methods: 232 male volunteers with mean age of 34 years (range 20-47) from 5 different regions of Ukraine (Zhytomyr, Ivano-Frankivsk, Kyiv, Poltava, and Kyiv city) were enrolled in cross-sectional studies of long term radiation effects on seminal plasma and sperm. All manipulations, analysis and classification of ejaculates were done accordingly to WHO recommendations.

Impact peculiarities of long-term gamma-irradiation with low-dose rate on the development of laboratory rats and their sperm production.

Objective: To study the influence of long-term gamma-irradiation with low dose rate (13×10-6 cGy/s) on the body, testes and ventral prostate, development of laboratory rats, as well as quantitative parameters of sperm production along with kinetic characteristics of spermatozoa.

Materials And Methods: The experiments were performed on laboratory white rats of 2,5 months in age. Animals were irradiated in gamma-field of "Ethalon" device in a dose range 0.

Variation of rabbit sperm physiologic parameters after the X-ray exposure.

Objective: To study the impact of X-irradiation on the morpho-functional characteristics of rabbit spermatozoa and the contents of fructose, L-carnitine, α-tocopherol, ascorbate and citrate in the animal sperm.

Materials And Methods: The total body irradiation of rabbits (Soviet Shinchilla) was performed at the RUM-17 device in the dose range of 1.0-7.

[Using method of DNA microelectrophoresis for the assessment of genotoxic effects of mutagens in cultured human hemopoietic cells].

Formation of single- and double strand breaks in DNA which may be discovered by microelectrophoresis in agarose gel is one of the criterion of genetical lesions in cells as a result of apoptosis or of genotoxic agent action. Genotoxic action of nickel chloride at the level of DNA of the individual cells in the initial culture of human embryonic haemopoietic cells was studied. It has been shown that about 2% cells in the studied in vitro populations were in the apoptosis state.

[Outbreak of giardiasis at a preschool children establishment in the town of Perm'].

In November 2000, an outbreak of Giardiasis intestinalis with aqueous transmission was recorded in one of the microdistricts of Perm. Thirty six children aged 1-7 years fell ill, 2 parasitic carriers were identified among the staff. The diagnosis was parasitologically verified in all children with clinical manifestations.