The nuclear DNA and RNA distribution in Pelomyxa spp. (Amoebozoa, Archamoebae, Pelobiontida) revealed by a simple-to-use DAPI/pyronin staining method.

The genus Pelomyxa includes 15 species of anaerobic Archamoebae with remarkable diverse nucleoplasm morphology. Nuclear structures, like chromatin and nucleoli, of several members of the genus was previously identified only based on their ultrastructural similarity to typical structures of somatic cells of higher eukaryotes. Here, we explored an easy-to-use, one-step intravital staining method with DAPI and pyronin to distinguish between DNA and RNA structures in nuclei of unfixed cells of Pelomyxa belevskii and P.

New nucleolin-containing cytoplasmic bodies in an archamoebian protist Pelomyxa belevskii (Amoebozoa, Archamoebae, Pelobiontida).

The representatives of the archamoebian genus Pelomyxa are amoeboid anaerobic protists that inhabit fresh-water anoxic sediments, and most of them are usually multinucleate. The cytoplasm of these unicellular organisms is highly complicated and contains numerous vacuoles of different types, as well as a wide range of prokaryotic endocytobionts, agglomerations of glycogen, lipids, etc. Among the great variety of cytoplasmic structures in P.

Ciliates from the faeces of the free-ranging dromedary from Oman: Morphology and molecular phylogeny.

Ciliates Infundibulorium cameli from the faeces of the free-ranging dromedary from Oman were studied using a set of methods of the light and immunofluorescence microscopy and molecular phylogeny. With the use of molecular genetic methods, it was confirmed that the cysts found in the samples simultaneously with trophozoites actually belong to the species I. cameli.