TIP GROWTH OF NEUROSPORA CRASSA IN THE CONDITIONS OF THE RESOURCE DEFICIENCY: IMPAIRMENTS IN THE COHERENCE OF ELONGATION, BRANCHING, AND SEPTATION.

With the aid of intracellular fluorescent probes, peculiarities of elongation, branching, septation, and nucleus morphology have been studied in isolated 400-μm-long apical fragments of Neurospora crassa hyphae growing for several hours without the influx of the nutrient materials from the mycelium. We found that: 1) the diameter and growth rate of newly developing branches decreased as compared to intact mycelium (from 10-20 to 6.5 ± 0.

[Organization of mitochondria in the growing hyphae of Neurospora crassa].

In vivo fluorescent labeling of mitochondria in Neurospora crassa showed the concentration of filamentous mitochondria within 30 μm of apex in growing hyphae. These mitochondrial assemblies propagated forward with the elongation of hyphae, split and segregated as the growing tip bifurcated and formed de novo when new branches formed farther away from the apex. The efficiency of the mitochondria concentration in the apical 30 μm zone is related to the growth rate and identical in hyphae cultivated in glucose- and sorbitol-containing media.

[Protective role of the glutathione system in rat organs after administration of embiquine].

Dynamics of changes of the reduced glutathione contents, activity of glutathione transferase, glutathione reductase and gamma-glutamyl transpeptidase in rat organs at a single injection of embiquine in a dose of 1/2 LD50 has been studied. It was shown that injection of the alkylating antitumor preparation lead to the decrease of the reduced glutathione contents in the early period of intoxication together with compensatory increase of tripeptide concentrations 3 hours later in the liver tissue and 24 hours later in the kidney tissue. Embiquine caused the steady increase of the glutathione transferase activity in all the organs studied.

[Role of glutathione antiperoxide system in the mechanism of cytotoxic action of embichin].

Dynamics of changes in the peroxide level, contents of reduced and oxidized glutathione, activity of glutathione peroxidase and glutathione reductase in different organs of a rat under the influence of a single injection of embiquine in the dose of 1/2 DL50 was studied. Alkylating antitumor preparation was shown to cause the decrease of glutathione peroxidase and glutathione reductase activity. Activation glutathione antiperoxide system decreased cytotoxic effects of embiquine by prevention of lipoperoxid accumulation in the liver in the nearest periods of investigation after injection of the preparation, and in the kidney and spleen--during the whole period of investigation.