Publications by authors named "L Toribio"
The simultaneous separation of the enantiomers of six anticoagulant rodenticides, derived from 4-hydroxycoumarin, has been studied in this work. Ten different stationary phases (zwitterionic, Pirkle-type, polysaccharides and macrocyclic antibiotics derivatives) were evaluated by using supercritical fluid chromatography coupled to two different detectors (circular dichroism and mass spectrometry-single quadrupole). The effect of the type of organic modifier and temperature on the chiral separation was investigated, and the best results were obtained with the column Regis S,S-Whelk-O1 at 25 °C when using a gradient elution program with methanol as organic modifier.
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- * A comprehensive review analyzed 169 records, focusing on 53 studies that evaluated the accuracy of various antibody and antigen tests using human serum or urine, revealing challenges in data interpretation due to variability in results.
- * Notably, a new multi-antigen print immunoassay showed high sensitivity and specificity for detecting both parenchymal and extraparenchymal cysts, while point-of-care tests also showed potential but need additional validation, particularly in resource-limited settings.
In the last decade, the instrumentation improvements in supercritical fluid chromatography (SFC) and the hyphenation to mass spectrometry (MS), have increased the SFC acceptance between scientists, becoming today a valuable tool in analytical chemistry. The unique selectivity, short analysis times, low consumption of organic solvents, and the greener mobile phase, have contributed to expanding its applicability which has led to an increase in the number of publications especially in the bioanalysis area. This work reviews the advantages and main applications of SFC in bioanalysis during the last 5 years.
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- - Neurocysticercosis is a significant parasitic disease, and diagnosing it often requires neuroimaging, which can be hard to access in poor rural areas.
- - A new, quick point-of-care (POC) test was developed to detect urinary antigens, improving diagnosis by identifying individuals who need further imaging.
- - The POC assay showed a 73.6% overall agreement with the standard Ag-ELISA, performing especially well in nonreactive and positive sample groups, making it a practical and noninvasive option for community screening.
Background: Antigen detection in Taenia solium cysticercosis confirms viable infection in the intermediate host (either pig or human). The reference B158/B60 monoclonal antibody (mAb)-based Ag-enzyme-linked immunosorbent assay (ELISA) has acceptable levels of sensitivity and specificity in human neurocysticercosis with multiple brain cysts, although its sensitivity is lower in cases with single brain cysts, whereas in porcine cysticercosis the assay specificity is affected by its frequent cross-reaction with Taenia hydatigena, another common cestode found in pigs. Our group has produced 21 anti-T.
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