Publications by authors named "L Tesson"
Copy number variations (CNVs) of the human 16p11.2 locus are associated with several developmental/neurocognitive syndromes. Particularly, deletion and duplication of this genetic interval are found in patients with autism spectrum disorders, intellectual disability and other psychiatric traits.
View Article and Find Full Text PDFCRISPR-Cas9 cleavage efficacy and accuracy are the main challenges gene editing faces, and they are particularly affected by the optimal formation of the ribonucleoprotein (RNP) complex. We used nano differential scanning fluorimetry, a label and immobilization-free assay, to demonstrate that an equimolar ratio of Cas9 and guide RNA (gRNA) is optimal for RNP complex formation. We almost achieved 50% of green fluorescent protein (GFP) to blue fluorescent protein (BFP) conversion using a biallelic homozygous GFP human induced pluripotent stem cell line, when 0.
View Article and Find Full Text PDFBackground: Regulatory T cells (Treg) in diverse species include CD4 and CD8 T cells. In all species, CD8 Treg have been only partially characterized and there is no rat model in which CD4 and CD8 FOXP3 Treg are genetically tagged.
Results: We generated a Foxp3-EGFP rat transgenic line in which FOXP3 gene was expressed and controlled EGFP.
Oral Delivery of miR-320-3p with Lipidic Aminoglycoside Derivatives at Mid-Lactation Alters miR-320-3p Endogenous Levels in the Gut and Brain of Adult Rats According to Early or Regular Weaning.
Int J Mol Sci
December 2022
Article Synopsis
- The study explored how delivering specific microRNAs from breastmilk affects the gut and brain development in young rats as they wean, focusing on a new transgenic rat line.
- Rats received an oral dose of miR-320-3p or miR-375-3p and were weaned either early or at the regular time, with various biological markers measured post-treatment and later.
- Results showed that the microRNAs influenced chromatin complexes and gene expression differently depending on the weaning timing, suggesting potential avenues for therapeutic interventions to mitigate long-term brain effects.
Introduction: Although the physiological role of the C-terminal hydrolase domain of the soluble epoxide hydrolase (sEH-H) is well investigated, the function of its N-terminal phosphatase activity (sEH-P) remains unknown.
Objectives: This study aimed to assess in vivo the physiological role of sEH-P.
Methods: CRISPR/Cas9 was used to generate a novel knock-in (KI) rat line lacking the sEH-P activity.