Evidence of inter-component recombination, intra-component recombination and reassortment in banana bunchy top virus.

Banana bunchy top virus (BBTV; family Nanoviridae, genus Babuvirus) is a multi-component, ssDNA virus, which causes widespread banana crop losses throughout tropical Africa and Australasia. We determined the full genome sequences of 12 BBTV isolates from the Kingdom of Tonga and analysed these together with previously determined BBTV sequences to show that reassortment and both inter- and intra-component recombination have all been relatively frequent occurrences during BBTV evolution. We found that whereas DNA-U3 components display evidence of complex inter- and intra-component recombination, all of the South Pacific DNA-R components have a common intra-component recombinant origin spanning the replication-associated protein gene.

First Record of Bacterial Crown Rot of Papaya (Carica papaya) Caused by an Erwinia papayae-Like Bacterium in the Kingdom of Tonga.

Symptoms resembling papaya bacterial crown rot (1,3) attributed to Erwinia papayae were first observed on 'Waimanalo' and 'Solo Sunrise' papaya on the island of Tongatapu, Kingdom of Tonga in July 2009. Spreading, dark green, water-soaked lesions formed on juvenile stem tissue and developed into a foul-smelling, wet rot that destroyed large sections of the stem. Coalescing, brown, angular, marginal, and intercostal lesions killed large areas of the lamina.