A new staining and evaluating procedure for protein gel electropherograms based on the pyrogallol red-molybdate complex.

A new method is reported for staining and evaluating gel electropherograms of proteins. With pyrogallol red-molybdate reagent the gel-embedded proteins are transformed into a derivative of blue colour. After destaining, the blue-coloured proteins are well visible against a colourless background and can be quantified by densitometry with high reliability.

Establishment of modern examining procedures for diagnosis of porphyrias.

Reversed-phase high-performance liquid chromatography together with fluorescence detection is suitable for the determination of urinary porphyrin carboxylic acid isomers, and Copro I and III, as well as Uro I and III porphyrin isomers are separated simultaneously.