A non-covalently bound redox indicator for electrochemical CRISPR-Cas12a and DNase I biosensors.

A rapid and accurate biosensor for detecting disease biomarkers at point-of-care is essential for early disease diagnosis and preventing pandemics. CRISPR-Cas12a is a promising recognition element for DNA biosensors due to its programmability, specificity, and deoxyribonuclease activity initiated in the presence of a biomarker. The current electrochemical CRISPR-Cas12a-based biosensors utilize the single-stranded DNA (ssDNA) self-assembled on an electrode surface and covalently modified with the redox indicator, usually methylene blue (MB).

Perceptions of teachers' responses to children's peer victimization: Agreement (and lack of agreement) among teachers and students.

Recent research has underscored the importance of teachers' responses to students' peer victimization as well as students' expectations for how their teacher responds to this victimization. However, little research has examined the extent to which teachers and their students have a shared understanding of their teacher's efforts, or lack of efforts, to manage peer victimization. This study addressed this issue using longitudinal data collected on 410 students (47.

Feeding Practices Used by Australian Parents of Young Children Living With Food Insecurity and Household Chaos.

Responsive feeding practices are crucial for developing healthy eating behaviours in children. However, chaotic households and financial stress may disrupt these practices. This cross-sectional study aimed to characterise feeding practices among Australian parents experiencing financial hardship.

Patterns of Simultaneous and non-Simultaneous Use of Cannabis and Alcohol Among American Indian Adolescents.

Background: American Indian (AI) adolescents report earlier initiation and higher rates of cannabis and alcohol use compared to their non-AI peers. Simultaneous cannabis and alcohol (SCA) use is increasingly common. A primary goal of our research was to identify profiles of cannabis and alcohol use, including SCA use, among AI adolescents using latent class analysis (LCA).

Frequency is not enough: Characterizing heterogenous patterns of cannabis use intensity among reservation-area American Indian youth.

Background: Reservation-area American Indian (AI) youth use cannabis at significantly higher rates than their national counterparts. This discrepancy is concerning, as cannabis use-particularly heavy use-can negatively impact adolescents' health. Studies primarily use frequency to classify cannabis use intensity; however, frequency alone may not fully capture heterogenous patterns of use.