Publications by authors named "L Spanoghe"

Sperm collection and cryopreservation are key technologies for assisted reproduction, with post-mortem sperm collection being the main tool to prevent the decline of endangered species. The present study describes post-mortem sperm collection and two cryopreservation protocols in beauty snakes (Elaphe taeniura), as a model for Colubridae. The vasa deferentia of 18 snakes were collected post-mortem and incubated for 30 min at room temperature to retrieve sperm by float up.

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Article Synopsis
  • * The study analyzed 3,090 ejaculates from 1,040 dogs of 157 breeds, revealing that most dogs were between 1 and 9 years old during collection, and a significant portion of the sperm samples remained unused or only partially used.
  • * Findings indicate a shift in breeding practices, with cryopreserved sperm primarily used for international shipping and 21.8% for local artificial insemination, highlighting the importance of this technique for preserving canine genetics. *
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The microbiome of the reproductive tract is an area of research in full development. Specifically, the microbiome may be involved in reproductive health, disease, and pregnancy outcomes, as has been shown in humans and animals, including dogs. The aim of the present review was to summarize current knowledge on the microbiome of the canine reproductive tract, to expose the controversial role that some bacterial agents may play in canine subfertility, and to highlight future research perspectives.

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Article Synopsis
  • The study explores the microbiota in the semen of male dogs using DNA sequencing, focusing on factors like ejaculate fraction, sperm quality, and living environment.
  • * It collected sperm-rich and prostatic fractions from healthy dogs with different sperm qualities and analyzed the bacterial composition.
  • * Findings indicated that while the living environment affects bacterial diversity, the ejaculate fraction and sperm quality do not significantly change the microbiota composition.
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The preventive action of ringworm vaccine LTF 130 was studied in calves. A number of these calves were found to develop ringworm despite vaccination when they came into contact with infected animals during the build-up of immunity. The incidence of ringworm was reduced when the animals were vaccinated at an early age or when the dosage was increased on vaccination later in life.

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