Reflex zone stimulation reduces ventilation inhomogeneity in cystic fibrosis: A randomised controlled cross-over study.

Background: The reflex zone stimulation technique (RST) activates complex motor responses and has a positive impact on the locomotor system. This technique may also indirectly affect breathing; however, the use of this technique as adjunct of the treatment of cystic fibrosis (CF) has not yet been characterised.

Methods: We performed a randomised controlled single-centre interventional trial to evaluate the short-term effects of RST on lung function in 21 paediatric CF patients with normal baseline spirometry.

[The differentiation of specimiens of Vibrio parahaemolyticua and Vobrio alginolyticus].

Vibrio parahaemolyticua and Vobrio alginolyticus are phylogenetically closely-related species. They have common ecological niches, same cultural features and similar biochemical characteristics. The phenotype variability and taxonomy similarity of strains of these species impedes differentiation of Vibrio parahaemolyticua and Vobrio alginolyticus according biochemical characteristics.

[Collection of Vibrio parahaemolyticus species members: phenotypic and genotypic characteristics].

Aim: Formation of Vibrio parahaemolyticus collection according to modern methodical opportunities and understanding of causative agent biology.

Materials And Methods: Traditional biochemical tests and PCR-testing of species-specific genes were used to confirm species membership. Catalase, DNAse, proteolytic and tweenase activity was determined by common methods.

[O3:K6 serogroup vibrio parahaemolyticus - the causative agent of food toxic infection outbreaks in Primorsky region of Russian federation].

Aim: Comparative evaluation of biological properties of parahemolytic vibrios that had determined outbreaks and sporadic cases of food toxic infection in Primorsky Region in 2012 and previous years. Materials AND METHODS: 40 clinical strains of Vibrio parahaemolyticus isolated in 2012 were studied in comparison with 62 strains from this region that had been characterized by us previously. Virulence was evaluated by a complex method: hemolytic activitywas determined in Kanagawa test (KT), urease - in Kristensen medium.

[The development of complex technique of evaluation of virulence of parahemolytic vibrio].

The article deals with results of studying parahemolytic vibrio separatedfrom different sources according their phenotype and genotype attributes associated with virulence. In certain cases the mismatch of results of Kanagava tests and polymerase chain reaction test of gene tdh was established. The need in virulence complex evaluation is substantiated.