Different effects of (CIS+TRANS) 1,3-dichloropropene in renal cortical slices derived from male and female rats.

Nephrotoxic effects of 1,3-dichloropropene (cis and trans isomers mixture) was investigated in vitro by means of renal cortical slice model in male and female rats, including treatment with metabolism modifiers as an inducer of cytochrome P-450 1A class (beta-naphthoflavone), a reduced glutathione depleting (DL-buthionine-[S,R]-sulfoximine), an inhibitor of gamma-glutamyl-transferase (AT-125) and inhibitor of cysteine conjugate beta-lyase (aminooxiacetic acid). Dose-dependent decrease of p-aminohippurate uptake was observed in male renal cortical slices. Only the high doses (3.

Influence of hormone status on enzymes released from renal cortical slices of Wistar rats.

Release of some cytosolic (aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase) and brush border (gamma-glutamyltransferase and alkaline phosphatase) enzymes from renal cortical slices was studied in vitro. Renal cortical slices were prepared freehand from 3-month-old male and female Wistar rats of different hormonal status. Some male and female rats were castrated at 1 month of age and a portion of castrated males and of naive males and females were s.

Short Communication: Urinary excretion of glutamine transaminase K as an early index of mercuric chloride-induced nephrotoxicity.

Abstract The possibili that urinary glutamine transaminase K activity might be a marker of a proximal tubule segment-specific response to mercuric chloride was investigated in male rats after a single i.p. injection in time-course and dose-response experiments.

Mechanism of sex-related differences in nephrotoxicity of 1.2-dichloropropane in rats.

The contribution of testosterone to the nephrotoxic effects of 1,2-dichloropropane (DCP) was assessed by a series of castration and sex hormone replacement experiments on Wistar rats. The nephrotoxic action of DCP was evaluated by measuring the accumulation of organic anion and release of aspartate aminotransferase into the incubation medium using a renal cortical slice model. Our data show that sex, castration, and testosterone pretreatment are factors that influence the effect of DCP on renal cortical slices of rats Males appear to be more sensitive to nephrotoxic effects of DCP than females, male castration prevents the nephrotoxic effects of DCP, and pretreatment of females and castrated males with testosterone increases the susceptibility to DCP.

Biological monitoring of cadmium exposure: reliability of spot urine samples.

Concentration-dilution of spot urine samples is a shortcoming of the biological monitoring of industrial xenobiotics. To ascertain whether the adjustment of urinary cadmium measured in spot samples is appropriate, urine samples were taken three times, once a week for 3 successive weeks, from 25 welders employed in the manufacture of jewelry (total 75 samples). Cadmium, creatinine, specific gravity, total urinary solutes, urinary volume and urinary flow rate were measured in 12-h collections and in spot samples taken immediately afterwards.