Maternity Care Providers' Experiences with Providing Information on Newborn Bloodspot Screening During Pregnancy: A Dutch Survey Study.

Newborn bloodspot screening (NBS) aims to detect treatable disorders in newborns to offer early interventions. According to the official Dutch national NBS guidance, parents in the Netherlands should be informed about NBS during pregnancy by maternity care providers (MCPs), providing two leaflets and oral information. This study investigated what, how, and when information about NBS is given during pregnancy according to Dutch MCPs.

Human-robot interactions and experiences of staff and service robots in aged care.

The rise of robotics in aged care is transforming how older adults are cared for, addressing staff shortages and workload. Daily interactions with staff and residents highlight an urgent need to better understand and improve human-robot interactions. This study investigates how 34 staff interacted with 10 service robots in a regional Australian aged care facility over 5 years, focusing primarily on their experience with service robots that transported meals and laundry.

Evaluation of the Cow's Milk and Hen's Egg Ladder in Daily Practice: A Parent Perspective.

Evaluation of the cow's milk (CM) and hen's egg (HE) ladder to improve medical care of children with CM and/or HE allergy. Through an anonymous retrospective online questionnaire, we investigated parent perspectives regarding the therapy option of a CM and HE ladder in children with CM and/or HE allergy. Of the 54 families contacted, 29 took part in the study.

An exploratory study on the effect of rescuer team size on basic and advanced life support technical skills in a high-fidelity simulation of canine cardiopulmonary arrest.

Objective: To evaluate the effect of rescuer team size on objective skill measures of basic life support (BLS) and advanced life support (ALS) using high-fidelity canine CPR simulation.

Design: Prospective, experimental study.

Setting: Veterinary clinical simulation center.

Helical transition of the bridge helix of Cas12a is an allosteric regulator of R-loop formation and RuvC activation.

CRISPR-Cas12a is widely used for genome editing and biomarker detection since it can create targeted double-stranded DNA breaks and promote non-specific DNA cleavage after identifying specific DNA. To mitigate the off-target DNA cleavage of Cas12a, we previously developed a Cas12a variant (FnoCas12a ) by introducing double proline substitutions (K969P/D970P) in a conserved helix called the bridge helix (BH). In this work, we used cryogenic electron microscopy (cryoEM) to understand the molecular mechanisms of BH- mediated activation of Cas12a.