Publications by authors named "L Rios-Solis"
Functional yogurt, renowned for its enhanced nutritional profile and potential health benefits, has emerged as a promising functional food. This review meticulously examines the nutritional composition of functional yogurt, highlighting its enriched content of probiotics, prebiotics, synbiotics, antioxidants, vitamins, minerals, proteins, and other bioactive compounds, which contribute to its health-promoting properties. Functional yogurt has positively affected digestive health, immune function, metabolic health, and mental well-being.
View Article and Find Full Text PDFIn the quest for innovative cancer therapeutics, paclitaxel remains a cornerstone in clinical oncology. However, its complex biosynthetic pathway, particularly the intricate oxygenation steps, has remained a puzzle in the decades following the characterization of the last taxane hydroxylase. The high divergence and promiscuity of enzymes involved have posed significant challenges.
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- - The study focused on optimizing the production and recovery of the anticancer drug Taxol (paclitaxel) from vascular stem cells of Taxus baccata, using in situ product recovery (ISPR) techniques with various resin beads and methyl jasmonate as an elicitor.
- - The optimal combination of resin beads increased the paclitaxel yield significantly, producing 234 ± 23 mg of paclitaxel per kg of fresh-weight cells, which was 13 times higher than the control group that didn't use resins.
- - Additionally, using ISPR lowered the levels of reactive oxygen species (ROS) in the cultivations, which is linked to better cell growth and product synthesis, leading to
Article Synopsis
- Microbial cell factories are being explored for producing high-value natural products sustainably, but recovering these products poses a major cost challenge.
- This study developed new 3D-printed hydrophobic adsorbents using benzyl methacrylate (BEMA) and butyl methacrylate (BUMA) as monomers, optimizing their pore structure with various porogens.
- The most effective adsorbent (BEMA40) demonstrated comparable taxadiene recovery to commercial options, showing that 3D printing can create customizable materials that enhance product recovery in microbial cultivation processes.
has been conveniently used to produce Taxol® anticancer drug early precursors. However, the harmful impact of oxidative stress by the first cytochrome P450-reductase enzymes (CYP725A4-POR) of Taxol® pathway has hampered sufficient progress in yeast. Here, we evolved an oxidative stress-resistant yeast strain with three-fold higher titre of their substrate, taxadiene.
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