Semi-quantitative scoring criteria based on multiple staining methods combined with machine learning to evaluate residual nuclei in decellularized matrix.

The detection of residual nuclei in decellularized extracellular matrix (dECM) biomaterials is critical for ensuring their quality and biocompatibility. However, current evaluation methods have limitations in addressing impurity interference and providing intelligent analysis. In this study, we utilized four staining techniques-hematoxylin-eosin staining, acetocarmine staining, the Feulgen reaction and 4',6-diamidino-2-phenylindole staining-to detect residual nuclei in dECM biomaterials.

Impact of sleep disruptions on gray matter structural covariance networks across the Alzheimer's disease continuum.

Background: This study explores the impact of sleep disturbances on gray matter structural covariance networks (SCNs) across the Alzheimer's disease (AD) continuum.

Methods: Amyloid-negative participants served as controls, whereas amyloid positive (A+) individuals were categorized into six groups based on cognitive status and sleep quality. SCNs for the default mode network (DMN), salience network (SN), and executive control network (ECN) were derived from T1-weighted magnetic resonance images.

A Manta Ray-Inspired Biosyncretic Robot with Stable Controllability by Dynamic Electric Stimulation.

Biosyncretic robots, which are new nature-based robots in addition to bionic robots, that utilize biological materials to realize their core function, have been supposed to further promote the progress in robotics. Actuation as the main operation mechanism relates to the robotic overall performance. Therefore, biosyncretic robots actuated by living biological actuators have attracted increasing attention.

Targeting ABCD1-ACOX1-MET/IGF1R axis suppresses multiple myeloma.

Multiple myeloma (MM) remains an incurable hematological malignancy that necessitates the identification of novel therapeutic strategies. Here, we report that intracellular levels of very long chain fatty acids (VLCFAs) control the cytotoxicity of MM chemotherapeutic agents. Inhibition of VLCFA biosynthesis reduced cell death in MM cells caused by the proteasome inhibitor, bortezomib.

A molecular sensor for selective recognition of Fe by a functional seven-nuclear Zn(II) cluster compound formed from a quinoline-modified half-salamo-type Precursor.

In this paper, we designed and synthesized a novel based on quinoline modification half-salamo-type Zn(II) complex([Zn(L)(μ-OCH)]·(ClO)), namely ZP. The structure of ZP was determined by X-ray single-crystal diffraction, in which the Zn(II) ions displayed in two different coordination modes, ultimately forming a highly symmetric heptanuclear structure. The Zn(II) complex probe modified by quinoline luminescent group has excellent luminescence properties and selectively recognizes Fe in aqueous solution.