Publications by authors named "L R Berghman"
The avian immune system responds to infection by expressing cytokines and chemokines. We hypothesized that the immune status of Typhimurium (ST) challenged neonatal broilers would differ from the uninfected treatment. The objective of this experiment was to evaluate 12 cytokines.
View Article and Find Full Text PDFObjective: Design and evaluate immune responses of neonatal foals to a mRNA vaccine expressing the virulence-associated protein A (VapA) of Rhodococcus equi.
Animals: Cultured primary equine respiratory tract cells; Serum, bronchoalveolar lavage fluid (BALF), and peripheral blood mononuclear cells (PBMCs) from 30 healthy Quarter Horse foals.
Methods: VapA expression was evaluated by western immunoblot in cultured equine bronchial cells transfected with 4 mRNA constructs encoding VapA.
Article Synopsis
- The study aimed to identify protective VP8 epitopes of equine rotavirus and test whether immunizing hens with synthetic peptides could produce effective neutralizing antibodies for foals.
- Researchers used white leghorn hens to generate high-titer anti-VP8 antibodies, which were then evaluated for their ability to neutralize the virus.
- The findings showed that administering hyperimmune egg yolk antibodies to foals with rotaviral diarrhea was safe and suggests potential for further study on their therapeutic benefits.
Article Synopsis
- The immune system uses a process called phagocytosis to destroy germs, but some germs, like the one that causes brucellosis, can trick this defense.
- This germ activates a special process that breaks down a protein (BLOS1) needed for moving germs to lysosomes, where they get destroyed.
- When certain cells or mice couldn't use this breakdown process, they were better at fighting off the infection, showing that this protein plays an important role in protecting against germs.
Electron beam (eBeam) inactivation of pathogens is a commercially proven technology in multiple industries. While commonly used in a variety of decontamination processes, this technology can be considered relatively new to the pharmaceutical industry. Rotavirus is the leading cause of severe gastroenteritis among infants, children, and at-risk adults.
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