Aza-[4 + 2]-cycloaddition of benzocyclobutenones into isoquinolinone derivatives enabled by photoinduced regio-specific C-C bond cleavage.

The activation of C-C bond of benzocyclobutenones under mild reaction conditions remains a challenge. We herein report a photoinduced catalyst-free regio-specific C1-C8 bond cleavage of benzocyclobutenones, enabling the generation of versatile ortho-quinoid ketene methides for aza-[4 + 2]-cycloaddition with imines, which offers a facile route to isoquinolinone derivatives, including seven family members of protoberberine alkaloids, gusanlung A, B, D, 8-oxotetrahydroplamatine, tetrahydrothalifendine, tetrahydropalmatine, and xylopinine. Furthermore, the catalytic enantioselective version of this strategy is also realized by merging synergistic photocatalysis and chiral Lewis acid catalysis.

The FvABF3-FvALKBH10B-FvSEP3 cascade regulates fruit ripening in strawberry.

Fruit ripening is a highly-orchestrated process that requires the fine-tuning and precise control of gene expression, which is mainly governed by phytohormones, epigenetic modifiers, and transcription factors. How these intrinsic regulators coordinately modulate the ripening remains elusive. Here we report the identification and characterization of FvALKBH10B as an N-methyladenosine (mA) RNA demethylase necessary for the normal ripening of strawberry (Fragaria vesca) fruit.

Robust Immobilization and Activity Preservation of Enzymes in Porous Frameworks by Silica-Based "Inorganic Glue".

The development of novel methods to enhance enzyme-carrier interactions in situ, at a feasible cost, and on a large scale is crucial for improving the stability and durability of current immobilized enzyme systems used in industrial settings. Here, a pioneering approach termed "silica-based inorganic glue" is proposed, which utilizes protein-catalyzed silicification to fix enzyme within porous matrix while preserving enzyme activity. This innovative strategy offers several key benefits, including conformational stabilization of enzymes, improved interactions between enzymes and the matrix, prevention of enzyme leakage, and mitigation of pore blocking.

Light quality regulates growth and flavonoid content in a widespread forest understorey medicinal species Georgi.

Introduction: Introduction: Light is not only essential for plant photosynthesis and growth, but also acts as a signal to regulate its secondary metabolism. Despite the influence of light quality on the yield and flavonoid compounds in commercial crops is well-documented, its role in regulating wild understorey species, particularly medicine plants whose flavonoid biosynthesis driven by multiple spectral regions of canopy sunlight, is less understood.

Methods: To address it, we conducted a light-quality manipulation experiment on Georgi, a widespread understorey medicinal species, with light-emitting diodes (LED).

Regulation of epidermal barrier function and pathogenesis of psoriasis by serine protease inhibitors.

Serine protease inhibitors (Serpins) are a protein superfamily of protease inhibitors that are thought to play a role in the regulation of inflammation, immunity, tumorigenesis, coagulation, blood pressure and cancer metastasis. Serpins is enriched in the skin and play a vital role in modulating the epidermal barrier and maintaining skin homeostasis. Psoriasis is a chronic inflammatory immune-mediated skin disease.