High detection rate of parasitic load by qPCR targeting 18S rDNA in blood of patients with active leishmaniasis lesions.

This study aimed to standardize qPCR techniques using these molecular markers kDNA and 18S rDNA across three sample types: peripheral blood, guanidine-treated blood, and tissue. The secondary objective is to evaluate the performance of 18S rDNA target in samples from 46 patients with confirmed tegumentary leishmaniasis. After obtaining the standard curve from reference strains with Leishmania, qPCR curves were standardizations and the Cts results of the patient samples were described using abstract measures.

Nanoarchitectonics for Advancing Bone Graft Technology: Integration of Silver Nanoparticles Against Bacteria and Fungi.

Silver nanoparticles have garnered significant attention for their antimicrobial applications. The aim of this study was to develop and characterize a silver nanoparticle-enhanced bone graft and assess its antimicrobial and antibiofilm activities. Bone granules from bovine cancellous femur were impregnated with silver nanoparticles (50 nm).

Combined therapeutic option for NDM-producing Serratia Marcescens - an in vitro study from clinical samples.

Background: Treating NDM-producing bacteria poses a significant challenge, especially for those bacteria inherently resistant to polymyxin, such as Serratia marcescens, necessitating combined therapies.

Objective: To assess in vitro the synergistic effect of different antimicrobial combinations against NDM-producing S. marcescens.

Evaluation of Tissue Biocompatibility of Calcium Silicate-based and Epoxy Resin-based Sealers.

Introduction: Calcium silicate-based sealers are an alternative to be used into root canal, mainly to their biological properties. However, some biological parameters need to be determined in an animal research model. So, the aim of the present study was to evaluate the tissue biocompatibility of a calcium silicate-based sealer (EndoSequence BC Sealer) and an epoxy resin-based sealer (AH-Plus).