Publications by authors named "L Puttagunta"
Article Synopsis
- - The study focuses on pulmonary neuroendocrine cells (PNEC), which are important yet rare cells in the airway that may influence allergic asthma, and introduces a new in vitro model to study their functions in response to allergens like house dust mite (HDM).
- - Researchers created PNEC cultures from human stem cells and primary bronchial cells, exposing them to various stimuli (HDM, Bergamot oil, and LPS) to measure changes in gene expression and neuropeptide release.
- - Findings showed that HDM specifically triggered an increase in the CALCB gene and the release of the neuropeptide CGRP, highlighting PNEC's role in the neuroendocrine response to allergens, which can
Calcium binding protein, spermatid associated 1 (CABS1) is a protein most widely studied in spermatogenesis. However, mRNA for CABS1 has been found in numerous tissues, albeit with little information about the protein. Previously, we identified CABS1 mRNA and protein in human salivary glands and provided evidence that in humans CABS1 contains a heptapeptide near its carboxyl terminus that has anti-inflammatory activities.
View Article and Find Full Text PDFHistological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton.
Methods Mol Biol
January 2022
Histochemical analysis is an indispensable technique in the field of biology used routinely to characterize pathologies of interest throughout the system. This chapter provides the craniofacial biologist with an introduction to tissue harvesting, embedding, and sectioning as well as a toolkit of useful stains for stromal/mesenchymal tissues including bone and cartilage. Techniques are tailored to decalcified, paraffin-embedded mouse tissue; however, these methods are applicable under a broad range of conditions.
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