The estimation of fructosamine and HbAlc in pregnant women with diabetes mellitus.

Fructosamine, HbAlc, glucose, albumins and total proteins were estimated in 40 healthy pregnant women and 80 pregnant women with insulin dependent diabetes mellitus. Fructosamine was estimated by the NBT method with "Fructosamine test" commercially available kit on Technicom automatic analyser RA-1000. Glucose was determined on Beckman glucose analyser.

[Lecithin in the amniotic fluid in the determination of fetal lung maturity].

A prospective study involved 90 patients divided into groups of high risk and risk pregnancies, which matched in age, parity and gestational age. Two methods for the amniotic fluid phospholipid analysis were compared. The value of lecithin was measured by the two phase UV enzyme method and the lecithin/sphingomyelin (L/S) ratio was determined by Gluck's method.

[Estrotec--a new immunological test for the study of estrogen in pregnancy].

For analysing estrogen in the urine of pregnant women an immunological method based on the principle of hemagglutination inhibition is applied. A total of 45 normal pregnant women were examined to check normal values, along with 30 pregnant women with EPH gestosis. Urine estrogen determinations were made concurrently by an immunological (Estrotec) and a chemical (Ittrich) method.

[Immunological determination of alpha-fetoproteins in pregnancy].

In 53 normal pregnant women the alphafetoprotein value (AFP) was determined by two parallel methods: immunological and radioimmunological. The results of this comparative analysis have shown a high significance, the coefficient o the linear correlation r being 1.0002 and the level of agreement obtained by the t test amounting to P less than 0.

[Enzyme immunoassay measurement of estrogens in EPH gestoses (author's transl)].

Investigations were made in 312 healthy gravidas, from 20 to 40 weeks of gestation, in order to establish normal values of serum and urinary estrogens. The study also included 210 gravidas with EPH gestosis classified into three groups according to symptomatology: mild EPH gestosis (98), moderate EPH gestosis (70), and severe EPH gestosis (42). Mild cases did not show any significant deviations from normal serum and urinary estrogen values.