Publications by authors named "L Perin"
Article Synopsis
- - Fasting-mimicking diets (FMD), specifically a low-salt version (LS-FMD), were tested in rats with kidney damage and were found to restore normal kidney function and structure by inducing a nephrogenic gene program.
- - LS-FMD activated pathways that promote cell reprogramming in the kidney, specifically targeting podocytes, which play a critical role in kidney health.
- - A pilot study in patients with chronic kidney disease showed that FMD cycles improved kidney function and reduced protein levels in urine, indicating potential for further research in treating progressive kidney diseases.
Article Synopsis
- Personalized disease models are essential for testing treatments on diseased cells, particularly innovative therapies, and this study focuses on extracellular vesicles (EVs) from kidney progenitor cells (nKPCs) as a potential treatment for steroid-resistant nephrotic syndrome in children.
- Urinary podocytes from affected patients were treated with nKPC-EVs and compared to standard drugs, showing that nKPC-EVs significantly reduced permeability in podocytes, while standard treatments had limited effectiveness.
- RNA sequencing identified the upregulation of two genes, SUMO1 and SENP2, linked to the treatment's effectiveness, suggesting that the SUMOylation pathway plays a crucial role in the therapeutic impact of nKPC-EVs
We study, through molecular dynamics simulations, three aqueous solutions with one lysozyme protein and three different concentrations of trehalose and dimethyl sulfoxide (DMSO). We analyze the structural and dynamical properties of the protein hydration water upon cooling. We find that trehalose plays a major role in modifying the structure of the network of HBs between water molecules in the hydration layer of the protein.
View Article and Find Full Text PDFObjective: To learn the strategies used regarding underreporting of pesticide use in rural areas.
Methods: A qualitative study was carried out in eight primary healthcare units in rural areas and two emergency care units in a municipality in southern Brazil. Data collection took place in 2023 through interviews.
Here, we used digital spatial profiling (DSP) to describe the glomerular transcriptomic signatures that may characterize the complex molecular mechanisms underlying progressive kidney disease in Alport syndrome, focal segmental glomerulosclerosis, and membranous nephropathy. Our results revealed significant transcriptional heterogeneity among diseased glomeruli, and this analysis showed that histologically similar glomeruli manifested different transcriptional profiles. Using glomerular pathology scores to establish an axis of progression, we identified molecular pathways with progressively decreased expression in response to increasing pathology scores, including signal recognition particle-dependent cotranslational protein targeting to membrane and selenocysteine synthesis pathways.
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