Publications by authors named "L Pelkmans"
Nuclear speckles are membraneless organelles that associate with active transcription sites and participate in post-transcriptional mRNA processing. During the cell cycle, nuclear speckles dissolve following phosphorylation of their protein components. Here, we identify the PP1 family as the phosphatases that counteract kinase-mediated dissolution.
View Article and Find Full Text PDFArrayed CRISPR libraries extend the scope of gene-perturbation screens to non-selectable cell phenotypes. However, library generation requires assembling thousands of vectors expressing single-guide RNAs (sgRNAs). Here, by leveraging massively parallel plasmid-cloning methodology, we show that arrayed libraries can be constructed for the genome-wide ablation (19,936 plasmids) of human protein-coding genes and for their activation and epigenetic silencing (22,442 plasmids), with each plasmid encoding an array of four non-overlapping sgRNAs designed to tolerate most human DNA polymorphisms.
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- Deep single-cell multi-omic profiling is an innovative method to study drug resistance in relapsed or refractory acute myeloid leukemia (AML).
- The research combines various single-cell analyses and clinical data from 21 patients, revealing that treatment with both a hypomethylating agent and the Bcl-2 inhibitor venetoclax leads to reduced drug responsiveness compared to other treatment sequences.
- The study identifies both known and new resistance mechanisms, suggesting alternative therapies like the PLK inhibitor volasertib and highlighting the potential of targeting CD36 in treatment-resistant AML blasts.
Plant protoplasts provide starting material for of inducing pluripotent cell masses that are competent for tissue regeneration in vitro, analogous to animal induced pluripotent stem cells (iPSCs). Dedifferentiation is associated with large-scale chromatin reorganisation and massive transcriptome reprogramming, characterised by stochastic gene expression. How this cellular variability reflects on chromatin organisation in individual cells and what factors influence chromatin transitions during culturing are largely unknown.
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- Plasma membrane-associated platforms (PMAPs) form in specific areas of the plasma membrane through scaffolds like ERC1 and Liprin-α1, and their assembly affects cell movement and invasion.
- Silencing the kinase DYRK3 in invasive breast cancer cells reduces their motility and capacity to invade, while increasing DYRK3 levels also impacts movement negatively.
- DYRK3 overexpression leads to less stable lamellipodia by increasing phosphorylation of Liprin-α1, indicating that a balance between DYRK3 and Liprin-α1 is necessary for cell stability and movement in tumors.