Cryptococcus neoformans Database in Synthetic Biology Open Language.

Cryptococcus neoformans is the etiologic agent of cryptococcosis, a lethal worldwide disease. Synthetic biology could contribute to its better understanding through engineering genetic networks. However, its major challenge is the requirement of accessible genetic parts.

Comparison of Juvenile Feed Protocols on Growth and Spawning in Zebrafish.

Over the past 2 decades, zebrafish, , have become a mainstream laboratory animal model, yet zebrafish husbandry practices remain far from standardized. Feeding protocols play a critical role in the health, wellbeing, and productivity of zebrafish laboratories, yet they vary significantly between facilities. In this study, we compared our current feeding protocol for juvenile zebrafish (30 dpf to 75 dpf), a 3:1mixture of fish flake and freeze-dried krill fed twice per day with live artemia twice per day (FKA), to a diet of Gemma Micro 300 fed once per day with live artemia once per day (GMA).

Haematopoietic stem cell-dependent Notch transcription is mediated by p53 through the Histone chaperone Supt16h.

Haematopoietic stem and progenitor cells (HSPCs) have been the focus of developmental and regenerative studies, yet our understanding of the signalling events regulating their specification remains incomplete. We demonstrate that supt16h, a component of the Facilitates chromatin transcription (FACT) complex, is required for HSPC formation. Zebrafish supt16h mutants express reduced levels of Notch-signalling components, genes essential for HSPC development, due to abrogated transcription.

Body Condition Scoring for Adult Zebrafish ().

Body condition scoring (BCS) is a simple, rapid, noninvasive tool used to assess body condition in animals. In this study, we developed and validated a diagram-based BCS for adult zebrafish (), a popular research model. After receiving 20 min of hands-on training regarding the scoring system, 5 people each rated 95 adult zebrafish.

Simple, common but functional: biocompatible and luminescent rare-earth doped magnesium and calcium hydroxides from miniemulsion.

Nanostructured (d∼ 20-35 nm) and highly luminescent Ca(OH):Ln and Mg(OH):Ln (Ln = Eu, Sm, Tb, Mg(Ca)/Ln = 20 : 1 atomic) nanostructures were obtained in inverse (water in oil - w/o) miniemulsion (ME), by exploiting the nanosized compartments of the droplets to spatially confine the hydroxide precipitation in basic environment (NaOH). The functional nanostructures were prepared using different surfactants (Span80 (span) and a mixture of Igepal co-630 and Brij 52 (mix)) to optimise ME stability and hydroxide biocompatibility as well as tune the droplet sizes. X-Ray diffraction (XRD) analyses testify the achievement of a pure brucite-Mg(OH)-phase and pure portlandite-Ca(OH)-phase with a high degree of crystallinity.