Feasibility pilot of a novel coaching intervention to optimize cannabis use for chronic pain management among Veterans.

Introduction: Chronic pain is common among Veterans, some of whom use cannabis for pain. We conducted a feasibility pilot study of a novel coaching intervention to help Veterans optimize use of medical cannabis products for pain management (NCT06320470).

Methods: The intervention drew from scientific literature, consultation with cannabis experts, Veteran input via a Community Advisory Board, and tenets of motivational interviewing.

Engaging health system, service and consumer representatives in the co-design of a multi-criteria decision-making framework for commissioning overweight and obesity programs and services.

Background: Obesity is a multi-faceted problem that requires complex health system responses. While no single program or service is sufficient to meet every individual's needs, some criteria that increase the likelihood of program/service quality delivery to produce effective outcomes exist. However, although research on health commissioning is available internationally and is growing within the Australian context, no evidence exists of a multi-criteria decision-making framework to address the complexity required for effective commissioning of overweight and obesity early intervention and weight management programs or services.

Helical transition of the bridge helix of Cas12a is an allosteric regulator of R-loop formation and RuvC activation.

CRISPR-Cas12a is widely used for genome editing and biomarker detection since it can create targeted double-stranded DNA breaks and promote non-specific DNA cleavage after identifying specific DNA. To mitigate the off-target DNA cleavage of Cas12a, we previously developed a Cas12a variant (FnoCas12a ) by introducing double proline substitutions (K969P/D970P) in a conserved helix called the bridge helix (BH). In this work, we used cryogenic electron microscopy (cryoEM) to understand the molecular mechanisms of BH- mediated activation of Cas12a.

Diastolic Dysfunction and Survival in Patients with Preserved or Mildly Reduced Left Ventricular Ejection Fraction following Myocardial Infarction.

Background: Left ventricular ejection fraction (LVEF) is relatively indiscriminate for prognosis in patients with preserved or mildly reduced LVEF (>40%) following myocardial infarction (MI). This study sought to determine the value of guideline-based assessment of diastolic dysfunction (DD) in predicting long-term all-cause and cardiac mortality in patients with a first-ever MI and LVEF>40%.

Methods: A retrospective single centre study involving 2234 patients with a first-ever MI (STEMI or NSTEMI) with LVEF>40% was performed.